Invitrogen™ PKC theta Monoclonal Antibody (M217)

Catalog No.	Quantity
PIMA547677	100 μL

Catalog No. PIMA547677 Supplier Invitrogen™ Supplier No. MA547677

Description

Mouse Monoclonal Antibody

This antibody detects a 79 kDa* protein corresponding to the molecular mass of PKCtheta on SDS-PAGE immunoblots of human Jurkat cell lysates.

Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involve. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu. The different PKC isoforms vary in structure, subcellular localization, tissue distribution and response to extracellular signals. PKC beta II has been shown to be expressed, in part, in cardiac muscle, intestinal epithelium, kidney and pancreas. PKC beta II has been shown to be involved in cell cycle progression and cancer proliferation.

Specifications

• Antigen: PKC theta
• Applications: Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: M217
• Concentration: 0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: PRKCQ
• Gene Accession No.: Q02111, Q04759, Q9WTQ0
• Gene Alias: A130035A12Rik; AW494342; EC 2.7.11.13; kinase nPKC-theta; KPCT; MGC126514; MGC141919; nPKC-theta; PKC theta; PKC-0; Pkcq; PKCtheta; PKC-theta; PRKCQ; PRKCT; protein kinase C theta; protein kinase C theta type; protein kinase C, theta; RP11-563J2.1; si:ch211-239o15.1
• Gene Symbols: PRKCQ
• Host Species: Mouse
• Immunogen: Clone (M217) was generated from a recombinant mouse PKC theta protein that included amino acids residues in the N-terminal region. This sequence is conserved in human and rat PKC theta, and has low homology to other PKC family members.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 18761, 5588, 85420
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a