Invitrogen™ PKM2 Recombinant Rabbit Monoclonal Antibody (3H4L19), Alexa Fluor™ Plus 555, Invitrogen™

Catalog No.	Quantity
50-274-0591	20 μL
70-338-2RP555	50 μL

Catalog No. 50-274-0591 Supplier Invitrogen™ Supplier No. 703382RP55520UG

Description

Rabbit Recombinant Monoclonal Antibody

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser Filtration: 0.2 μm post-manufacturing filtered.

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.

Specifications

• Antigen: PKM2
• Applications: Western Blot
• Classification: Recombinant Monoclonal
• Clone: 3H4L19
• Concentration: 1.0 mg/mL
• Conjugate: Alexa Fluor Plus 555
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
• Gene: PKM
• Gene Accession No.: P14618
• Gene Alias: AA414905; AL024370; AL024424; ATP:pyruvate 2-o-phosphotransferase; CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; HEL-S-30; isozymes M1/M2; m2pk; muscle pyruvate kinase; OIP3; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; PK2; Pk-2; Pk3; Pk-3; PKLR; PKM; pkm.L; PKM12; PKM2; Pykm; pyruvate kinase; pyruvate kinase 2/3; pyruvate kinase 3; pyruvate kinase isozymes M1/M2; pyruvate kinase M1/2; pyruvate kinase M1/2 L homeolog; pyruvate kinase M1/2 L homeolog; pyruvate kinase, muscle; pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, liver and RBC; pyruvate kinase, muscle; TCB; THBP1; thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tu; tumor M2-PK; XELAEV_18018020mg
• Gene Symbols: PKM
• Host Species: Rabbit
• Immunogen: Peptides corresponding to human PKM2 [(aa476-aa493) (aa120-aa136)].
• Purification Method: Protein A
• Quantity: 20 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 5315
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG