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Invitrogen™ Platinum™ Direct PCR Universal Master Mix Non-distribution product as customer accommodation.

Invitrogen™ Platinum™ Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

$108.00 - $1500.00


Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
A44647100 Non-distribution product as customer accommodation.
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Invitrogen™
A44647100
100 Reactions
Each for $108.00
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A44647500 Non-distribution product as customer accommodation.
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Invitrogen™
A44647500
500 Reactions
Each for $450.00
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A44647200 Non-distribution product as customer accommodation.
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Invitrogen™
A44647200
5 x 500 Reactions
Each of 4 for $1,500.00
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Description

Description

  • Direct DNA amplification from various samples due to engineered, inhibitor-tolerant Platinum II Taq Hot-Start DNA Polymerase
  • Universal primer annealing temperature due to innovative buffer that enables primer annealing at 60°C
  • Superior specificity, sensitivity, and yields due to Platinum hot-start technology
  • Platinum Direct PCR Universal Master Mix is designed to work with a variety of samples of different origins such as animal, human, plant, insect, worm, and bacterial samples. The kit includes optimized reagents to achieve superior results. The quick lysis protocol enables efficient amplification from templates up to 8 kb in size and co-cycling of different length fragments. Samples in the lysis buffer can be stored for later use. Platinum Direct PCR Universal Master Mix is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.
  • Engineered Platinum II Taq DNA polymerase confers faster cycling and tolerance to reaction inhibitors originating from sample material. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.
  • Due to the unique composition of the Platinum Direct PCR Universal Master Mix, the annealing temperature of 60°C can be used for most primer pairs that follow the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. Different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.
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