Please call 1-800-766-7000 to place your order or try our site again later.
Invitrogen Platinum Hot Start PCR Master Mix (2X)
Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup
Manufacturer: Invitrogen 13000012
Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.
- High specificity and increased yields with antibody-mediated hot-start PCR
- Convenient room temperature reaction setup
- Versatile formulation for broad range of amplicons
Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The polymerase is activated in a temperature-dependent manner (at 94°C) during the start of PCR. Once dissociated from the antibodies, the Taq DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'3' exonuclease activity (but not 3'5' exonuclease activity).
Using Platinum Hot Start PCR Master Mix
Convenient Platinum Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. All you have to do is add template, specific primers, and water. Platinum Hot Start PCR Master Mix is supplied with a separate vial of Platinum GC Enhancer, formulated for specific amplification and improved yields of GC-rich targets. Use Platinum Hot Start PCR Master Mix in PCR applications such as genotyping, gene expression profiling, and next-generation sequencing
This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.
|5 kb or less|
|Each box contains:
• 1.25 mL Platinum Hot Start PCR 2X Master Mix
• 1.25 mL Platinum GC Enhancer
• 1.25 mL water, nuclease-free
Store at -20°C in a non-frost-free freezer.
|Built-In Hot Start|
|SuperMix or Master Mix|
|Platinum™ Taq DNA Polymerase|
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok