Invitrogen™ Platinum™ Tfi Exo(-) DNA Polymerase

Description

Platinum™ Tfi Exo(-) DNA Polymerase

Platinum™ Tfi Exo(-) DNA Polymerase is recombinant Tfi Exo(-) DNA Polymerase complexed with a proprietary antibody mix that inhibits polymerase activity at ambient temperature, allowing room-temperature reaction setup. Activity is restored after the initial denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' for increased specificity, sensitivity, and yield. Tfi Exo(-) DNA Polymerase is purified from E. coli. expressing cloned mutants of the Thermus filiformis DNA polymerase gene. This enzyme has 5'→3' polymerase activity, but lacks both 5'→3' and 3'→5' exonuclease activity for improved yield and robustness. Tfi Exo(-) DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer.

Applications
Platinum™ Tfi Exo(-) DNA Polymerase can be used in protocols that currently use Platinum™ Taq DNA Polymerase without modification. PCR performance is comparable to that of Platinum™ Taq in yield, specificity, fidelity, and robustness. Like Platinum™ Taq, Platinum™ Tfi Exo(-) DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3' ends of PCR products.

Specifications

• Concentration: 5X
• Content And Storage: Platinum™ Tfi DNA Polymerase (500 units total, at 5U/μL) is supplied with 5X Platinum™ Tfi Reaction Buffer (4 × 1.3 mL) and 50 mM Magnesium Chloride (1 mL). Store at -20°C (non-frost-free).
• GC-Rich PCR Performance: Low
• Polymerase: Tfi DNA Polymerase
• Reaction Speed: Standard
• Product Line: Platinum™
• Product Type: DNA Polymerase
• Quantity: 500 units
• Shipping Condition: Dry Ice
• Fidelity (vs. Taq): 1 X
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Reaction Format: Separate Components
• Size (Final Product): 1 kb or less
• Volume: 5.2 mL

For Research Use Only. Not for use in diagnostic procedures.