Invitrogen™ Podoplanin Recombinant Rabbit Monoclonal Antibody (JM31-21)

Catalog No.	Quantity
PIMA532617	100 μL

Catalog No. PIMA532617 Supplier Invitrogen™ Supplier No. MA532617

Description

Rabbit Recombinant Monoclonal Antibody

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.

Specifications

• Antigen: Podoplanin
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: JM31-21
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide; pH 7.4
• Gene: PDPN
• Gene Accession No.: Q62011, Q86YL7
• Gene Alias: 29kDa cytosolic podoplanin intracellular domain; Aggrus; CTA-520D8.1; E11; E11 antigen epitope; epithelial cell surface transmembrane protein antigen; glycoprotein 36; Glycoprotein 38; glycoprotein, 36-KD; GP36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); Ots8; OTS-8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26; PA2.26 antigen; PDPN; PICD; Podoplanin; PSEC0003; PSEC0025; pulmonary type I alveolar epithelial cell transmembrane differentiation marker; RANDAM-2; RP23-348F1.2; RTI140; RTI40; T1a; T1A2; T1A-2; T1alpha; T1-alpha; TI1A; Transmembrane glycoprotein E11; type I cell 40 kDa protein
• Gene Symbols: PDPN
• Host Species: Rabbit
• Immunogen: Synthetic peptide within Human Podoplanin aa 35-75.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 10630, 14726
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG