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Mirus Bio™ pOET1C_6xHis Transfer Plasmid

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MIR6151 20 μL
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Description

pOET1C_6xHis Transfer Plasmid for high-yield protein production in insect cells.

Baculovirus transfer plasmid designed for use with the flashBAC™ Baculovirus Expression System and TransIT™-Insect Transfection Reagent. The powerful AcMNPV polyhedrin promoter affords high-level gene expression in insect cells. A C-terminal 6xHis-tag fusion sequence is included for recombinant protein purification with Ni-NTA agarose columns and subsequent removal by thrombin cleavage.
  • Simple construction of recombinant virus when used with the flashBAC™ Baculovirus Expression System
  • Can be used with any baculovirus expression system that relies on recombination in insect cells at the polyhedrin locus
  • Can be propagated in DH5α or other general purpose E. coli strains
Vector Features:
  • Col E1 origin of replication
  • Ampicillin resistance gene for selection in E. coli
  • Multiple cloning site for insertion of the foreign gene in the correct frame and orientation
  • C-terminal 6xHis-Tag™ fusion sequence

Warranty and Services

1 Year

Specifications

Concentration 500 ng/μL, Sterile filtered in TE Buffer
Content And Storage Store at -20°C
Format Kit
For Use With (Application) Protein production in insect cells
Product Type Transfer Plasmid
Quantity 20 μL
Product Title
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