Invitrogen™ Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA146093

Catalog No. MA146093

Description

MA1-46093 reacts with DNA-directed RNA Polymerase II Largest Subunit in human, yeast and mouse samples. Expected to cross-react with Drosophilia melanogaster, hamster, S. pombe, and Arabidopsis thaliana due to sequence homology. MA1-46093 has been successfully used in Western Blot, Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, IHC (P) and Immunoprecipitation applications. The MA1-46093 immunogen is 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). This antibody detects both the phosphorylated and non-phosphorylated forms.

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

Specifications

• Antigen: Phospho-RNA pol II CTD (Ser5)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P08775, P21951, P24928
• Gene Symbols: POL2, POLR2A
• Immunogen: Chemically synthesized phospho-ser 5 YSPTSpPS (Human).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Yeast
• Product Type: Antibody
• Isotype: IgG1

• Applications: ChIP Assay, ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, RNA Immunoprecipitation, CUT&RUN, Immunocytochemistry
• Clone: 4H8
• Conjugate: Unconjugated
• Gene: POLR2A
• Gene Alias: 220kDa; AW321876; DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha 1, 180 kDa; DNA polymerase alpha 1, catalytic subunit; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha p180 subunit; DNA polymerase alpha subunit I; DNA polymerase epsilon catalytic subunit; DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; DUN2; EC 2.7.7.6; G7a/Bat6Hom; hRPB220; hsRPB1; I79_002751; I79_016081; MGC75453; N0825; NSX; p180; Pol II S2p; POL2; Pola; Pola1; POLR2; POLR2A; POLRA; polymerase (DNA directed), alpha 1; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD); Polymerase (RNA II (DNA directed), large polypeptide; polymerase (RNA) II (DNA directed) polypeptide A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; polymerase, alpha 1; RNA; RNA POL2; RNA polymerase II 1; RNA polymerase II carboxy-terminal domain; RNA polymerase II C-terminal domain; RNA polymerase II largest subunit; RNA polymerase II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; Rpb1; RPB220; RPBh1; Rpii215; RpIILS; RPO2; RPO21; Rpo2-1; RPOL2; SUA8; YNL262W
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 20020, 5430, 855459
• Content And Storage: -20°C
• Form: Liquid