Invitrogen™ POLR3A Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA521730

Catalog No. PIPA521730

Description

Recommended positive controls: IMR32, SK-N-AS. Predicted reactivity: Mouse (97%), Zebrafish (94%), Xenopus laevis (94%), Chicken (97%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.

Specifications

• Antigen: POLR3A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: POLR3A
• Gene Alias: 9330175N20Rik; ADDH; BC053071; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; DNA-directed RNA polymerase III subunit RPC1; HLD7; hRPC155; POLR3A; polymerase (RNA) III (DNA directed) polypeptide A; polymerase (RNA) III (DNA directed) polypeptide A, 155kDa; polymerase (RNA) III subunit A; RGD1305574; RNA polymerase III 155 kDa subunit; RNA polymerase III subunit A; RNA polymerase III subunit C1; RNA polymerase III subunit C160; RNA polymerase III subunit RPC155-D; RPC1; RPC155
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11128
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.43 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: O14802
• Gene Symbols: POLR3A
• Immunogen: Recombinant fragment corresponding to a region within amino acids 182 and 482 of POLR3A (Uniprot ID#O14802).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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