Invitrogen™ Porcine MIF ELISA Kit

Description

ELISA

Porcine MIF quantitates porcine MIF in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant porcine MIF.

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

Specifications

• Accession Number: cytokine; delayed early response protein 6; DER6; GIF; Glif; Glutathione-binding 13 kDa protein; glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MGC107654; MIF; MMIF; Phenylpyruvate tautomerase
• Assay Range: 16 to 4,000 pg/mL
• Assay Sensitivity: 16 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 397412
• Gene Symbol: Mif
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: MIF
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov