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  5. Invitrogen™ Porcine MIF ELISA Kit 

Invitrogen™ Porcine MIF ELISA Kit

Catalog No. ES18RBX5
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Description

ELISA

Porcine MIF quantitates porcine MIF in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant porcine MIF.

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number cytokine; delayed early response protein 6; DER6; GIF; Glif; Glutathione-binding 13 kDa protein; glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MGC107654; MIF; MMIF; Phenylpyruvate tautomerase
Assay Range 16 to 4,000 pg/mL
Assay Sensitivity 16 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 397412
Gene Symbol Mif
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target MIF
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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