Invitrogen™ Porcine MIF ELISA Kit

ELISA

Supplier:  Invitrogen™ ES18RBX10

Catalog No. 50-254-5302

Description

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

Specifications

• Accession Number: P80928
• Assay Sensitivity: 16 pg/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Mif
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hrs 20 mins

• Assay Range: 16 to 4,000 pg/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): 397412
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Target: MIF
• Total Assay Time: 4 hrs 45 mins