Invitrogen™ PPAR alpha Monoclonal Antibody (3B6/PPAR)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1822

Catalog No. MA1822

Description

MA1-822 detects peroxisome proliferator activated receptors (PPAR) alpha from human, mouse, and rat tissues. MA1-822 has been successfully used in Western blot, Gel Shift and immunoprecipitation procedures. By Western blot, this antibody detects a ~52 kDa protein which corresponds to PPAR alpha from mouse adipose tissue extract. This antibody detects some non-specific bands on NIH-3T3 cell lysates. The MA1-822 immunogen is purified recombinant PPAR alpha protein.

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.

Specifications

• Antigen: PPAR alpha
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: P23204, P37230, Q07869
• Gene Symbols: PPARA
• Immunogen: Purified recombinant PPAR alpha protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ChIP Assay, Flow Cytometry, Gel Shift, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 3B6/PPAR
• Conjugate: Unconjugated
• Gene: PPARA
• Gene Alias: 4933429D07Rik; AW742785; hPPAR; I79_022392; MGC2237; MGC2452; Nr1c1; nuclear hormone receptor; nuclear receptor; nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor alpha; peroxisome proliferative activated receptor, alpha; peroxisome proliferative activated receptor, alpha isoform 1; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3; peroxisome proliferator-activated receptor alpha; PPAR; PPAR ALPHA; Ppara; PPARALPHA; PPAR-alpha; proliferator-activated receptor alpha; transcription factor belonging to the nuclear receptor superfamily
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 19013, 25747, 5465
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid