Cytiva PreScission™ Protease

Catalog No.	Quantity
45-001-319	500U

Catalog No. 45-001-319 Supplier Cytiva Supplier No. 27084301

Description

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

• Description: A genetically engineered fusion protein consisting of human rhinovirus 3C protease and GST; Specifically cleaves between the Gln and Gly residues of the recognition sequence of LeuGluValLeuPheGln/GlyPro.
• Application: Facilitates removal of the protease by allowing simultaneous protease immobilization and cleavage of GST fusion proteins produced from the pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3
• Storage Buffer Components: 50mM Tris-HCl (pH 8.0), 150mM NaCl, 10mM EDTA, 1mM DTT, and 20% glycerol.
• Unit Definition: One unit will cleave >90% of 100μg of a test GST-fusion protein in 50mM Tris-HCl, 150mM NaCl, 1mM EDTA, 1mM DTT, pH 7.0 at 5°C for 16 hrs.

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Specifications

• Buffer: Elution Buffer: 50mM Tris HCl, 10mM reduced Glutathione pH 8; Cleavage Buffer: 50mM Tris HCl pH 8, 150mM NaCl, 10mM EDTA, 1mM DTT and 20% Glycerol
• Product Type: Fusion Protein Protease
• Quantity: 500U
• Content And Storage: -20°C/-80°C
• For Use With (Application): Facilitates removal of the protease by allowing simultaneous protease immobilization and cleavage of >90% of 100μg of a test GST-fusion protein produced from the pGEX-6P vectors pGEX-6P-1, pGEX-6P-2 and pGEX-6P-3