R&D Systems™ ProDots Human BMP-4 Recombinant Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The ProDots Recombinant Human BMP-4 Protein is derived from NS0. The ProDots Recombinant Human BMP-4 Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q53XC5
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 652
• Molecular Weight (g/mol): Observed M.W.: 22-25kDa, reducing conditions 37-41, non-reducing conditions, Theoretical M.W.: 13kDa (monomer)
• Quantity: 5 μg
• Source: Mouse myeloma cell line,NS0-derived human BMP-4 protein Ser293-Arg408
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU per 1μg of the protein by the LAL method.
• Gene Alias: BMP2B, BMP-2B, BMP2B1, BMP2BMCOPS6, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, OFC11, ZYME
• Common Name: BMP-4
• Gene Symbol: BMP4
• Biological Activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is typically 2.5-15.0ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: For a stock solution, reconstitute at 50-200 μg/mL in sterile 4 mM HCl, or simply roll ProDot™ directly into cell culture medium for immediate use.
• Expression System: Mouse myeloma cell line, NS0-derived Ser293 - Arg408
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain