R&D Systems™ ProDots Human Flt-3 Ligand Recombinant Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The ProDots Recombinant Human Flt-3 Ligand Protein is derived from Sf 21 (baculovirus). The ProDots Recombinant Human Flt-3 Ligand Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: AAA17999.1
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 2323
• Molecular Weight (g/mol): Predicted MW: 17.5kDa Observed MW: 17-30kDa, reducing conditions
• Quantity: 50 μg
• Source: Spodoptera frugiperda,Sf 21 (baculovirus)-derived human Flt-3 Ligand protein Thr27-Pro185
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: FL, FLG3L, Flt3 ligand, Flt3L, FLT3LG, fms-related tyrosine kinase 3 ligand, SL cytokine
• Common Name: Flt-3 Ligand
• Gene Symbol: FLT3LG
• Biological Activity: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3. The ED50 for this effect is typically 0.2-1ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot™ directly into cell culture medium for immediate use.
• Expression System: Spodoptera frugiperda, Sf 21 (baculovirus)-derived Thr27 - Pro185
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain