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R&D Systems™ ProDots Human IL-3 Recombinant Protein

Catalog No. PRD20350
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50 μg
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PRD20350 250 μg
PRD20310 50 μg
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Catalog No. PRD20350 Supplier R&D Systems™ Supplier No. PRD20350
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The ProDots Recombinant Human IL-3 Protein is derived from E. coli. The ProDots Recombinant Human IL-3 Protein has been validated for the following applications: Bioactivity.

Specifications

Accession Number Q6GS87
For Use With (Application) Bioactivity
Gene ID (Entrez) 3562
Molecular Weight (g/mol) Predicted MW: 15kDa Observed MW: 14kDa, reducing conditions
Quantity 250 μg
Source E. coli-derived human IL-3 protein Ala20-Phe152,with and without an N-terminal Met
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1μg of the protein by the LAL method.
Gene Alias Hematopoietic growth factor, IL3, IL-3MGC79398, interleukin 3 (colony-stimulating factor, multiple), interleukin-3, Mast cell growth factor, mast-cell growth factor, MCGF, MCGFMGC79399, MULTI-CSF, multilineage-colony-stimulating factor, Multipotential colony-stimulating factor, P-cell stimulating factor, P-cell-stimulating factor
Common Name IL-3
Gene Symbol IL3
Biological Activity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is typically 0.02-0.1ng/mL.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Reconstitution For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot directly into cell culture medium for immediate use.
Expression System E. coli-derived Ala20 - Phe152, with and without an N-terminal Met
Purity or Quality Grade >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
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