R&D Systems™ ProDots Human PDGF-BB Recombinant Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The ProDots Recombinant Human PDGF-BB Protein is derived from E. coli. The ProDots Recombinant Human PDGF-BB Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q6FHE7
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 5155
• Molecular Weight (g/mol): Observed M.W.: 12kDa, reducing conditions, Theoretical M.W.: 12.3kDa (monomer)
• Quantity: 5 μg
• Source: E. coli-derived human PDGF-BB protein Ser82-Thr190
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Common Name: PDGF-BB
• Gene Symbol: PDGFB
• Biological Activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is typically 1.5-6.0ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in sterile 4 mM HCl, or simply roll ProDot™ directly into cell culture medium for immediate use.
• Expression System: E. coli-derived Ser82 - Thr190
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain