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R&D Systems™ ProDots Human VEGF 165 Recombinant Protein

Catalog No. PRD29301
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50 μg
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PRD29301 5 μg
PRD29310 50 μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The ProDots Recombinant Human VEGF 165 Protein is derived from Sf 21 (baculovirus). The ProDots Recombinant Human VEGF 165 Protein has been validated for the following applications: Bioactivity.

Specifications

Accession Number NP_001165097
For Use With (Application) Bioactivity
Gene ID (Entrez) 7422
Molecular Weight (g/mol) Observed M.W.: 20-22kDa, reducing conditions 39-42kDa, non-reducing conditions, Theoretical M.W.: 19.2kDa (monomer)
Quantity 5 μg
Source Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VEGF protein Ala27-Arg191
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
Common Name VEGF
Gene Symbol VEGFA
Biological Activity Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 is typically 1.0-6.0ng/mL.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Reconstitution For a stock solution, reconstitute at 100 μg/mL in sterile PBS or (at 100-500 μg/mL in sterile 4 mM HCl. For immediate use, simply roll ProDot™ directly into cell culture medium.)
Expression System Spodoptera frugiperda, Sf 21 (baculovirus)-derived Ala27 - Arg191
Purity or Quality Grade >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
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