Applied Biosystems™ ProFlex™ PCR System, 2 x 384-well

Catalog No.	Warranty
44-840-77	Limited Warranty
A30163	3 years of total service coverage with instrument rapid exchange
50-257-6134	5 years of total service coverage with instrument rapid exchange
A30164	5 years of total service coverage with 3 PMs

Catalog No. 44-840-77 Supplier Applied Biosystems™ Supplier No. 4484077

Description

ProFlex™ PCR Systems are the latest in our line of Applied Biosystems™ thermal cyclers, providing the reliability and performance you've come to expect from Applied Biosystems instruments.

ProFlex™ PCR Systems are the latest in our line of Applied Biosystems™ thermal cyclers, providing the reliability and performance you've come to expect from Applied Biosystems instruments. By combining an extended warranty with your instrument purchase, you'll extend the life of your investment and receive the support you need to keep your instrument running at peak performance.

Each ProFlex PCR System Extended Warranty Package includes instrument configuration and control features, as well as extended warranty benefits, that adapt to your research needs:

• Interchange block types if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Receive fast repair from factory-trained and certified service engineers
• Minimize instrument downtime by troubleshooting with experienced Technical Support teams

For detailed instrument information and specifications, see the ProFlex PCR System (Cat. No. 4484075).

AB Repair Center (ABRC) Extended Warranty

Add additional years of warranty coverage to your existing manufacturer's limited warranty with the AB Repair Center (ABRC) extended warranty. With ABRC support, factory-trained engineers will maintain and repair your instrument at our remote service center. Just mail us your instrument and we'll maintain or repair it and ship it back to you within 3 weeks. All parts, labor, and shipping charges are included.

The ABRC extended warranty is ideal for customers in regulated environments that must maintain asset tag consistency to comply with regulated protocols.

Certain exclusions apply. Contact your Service and Support Representative for details at service.sales@thermofisher.com.

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Specifications

• Thermal Accuracy: ±0.25°C (35°C to 99.9°C)
• High-throughput Compatibility: High-throughput Compatible
• Thermal Uniformity: <0.5°C (20 sec. after reaching 95°C)
• Reaction Speed: Fast, Standard
• Dimensions: 22 x 13 x 10.6 in. (56.5 x 33 x 27 cm)
• Capacity: 768 x 20 μL Tubes, 2 x 384-well Plate
• Voltage: 100/240 V
• Product Line: ProFlex
• Display Type: 8.4 in. Color TFT LCD Touchscreen
• Product Type: Thermal Cycler
• Warranty: Limited Warranty
• Thermal Range: 0°C to 100°C
• For Use With (Application): PCR
• Programs: Auto re-start (after Power Outages), Program Overwrite Protection
• User Interface: USB, WiFi, Cloud, Ethernet
• Max. Ramp Rate: 3°C/sec. (Block), 1.6°C/sec. (Sample)
• Quantity: 1 Instrument
• Memory: USB and On-board
• Weight: 45 lb. (20.4 kg)
• Block Configurations: 2 x 384-well Isothermal Blocks

Frequently Asked Questions (FAQs)

My tubes are melting. What is wrong?

This can happen if the wrong plastics are used, or if the tubes are not being used with the correct tray/retainer set. Check the manual for directions on appropriate loading of the sample. Also, you can check for compatible plastics from here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html).

Veriti Thermal Cycler - Loading Samples (p. 3-5 of the manual)
ProFlex PCR System - Loading Samples (p. 25 of the manual)
2720 Thermal Cycler - Loading Samples (p. 3-5 of the manual)

How do I program an infinite hold in the ProFlex PCR System?

To add an infinite hold, select the infinity symbol for program.

How do the VeriFlex Blocks work on the ProFlex PCR System?

In the ProFlex PCR System, the 96-well and 3 x 32-well blocks feature innovative VeriFlex Blocks for enhanced PCR functionality. Six separate Peltier blocks (2 on each 32-well block for 3 x 32-well) provide maximal versatility and flexibility and offer two key benefits:

1.PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
2.Run more experiments: the six Peltier blocks can also be used to run up to six different annealing temperatures in the same run.

What is the Auto Delta feature on the ProFlex PCR System?

The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.

What is the ramp rate of the ProFlex PCR System?

The 96-well and 3 x 32-well system have a max block ramp rate of 6.0 degrees C/sec and the dual 96-well and dual flat blocks have a max block ramp rate of 2.0 degrees C/sec.

What types of plastics can I use with the ProFlex PCR System?

The plates and tubes will be specific to the block type. Please refer to our PCR Tubes, Plates & Accessories Plastics Selection Guide (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html) for a list of compatible plastics, based on your block type.

What types of blocks are available on the ProFlex PCR System?

The ProFlex PCR System has 4 different blocks that can be changed with the flip of a switch, including the first-of-its-kind 3 x 32-well independent block. This block allows up to 3 experiments to be run simultaneously, completely independently of each other and without interference. A dual 96-well block is available for your high throughput sequencing needs and a dual flat block is available to support OpenArray plate technology for genotyping analysis on the QuantStudio 12K Flex Real-Time PCR System.

What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

How can I monitor my thermal cycler remotely without being on the Cloud?

The Thermal Cycler Fleet Control Software allows monitoring of the following instruments from within a firewall:
SimpliAmp Thermal Cycler
ProFlex PCR System
Veriti Thermal Cycler
VeritiPro Thermal Cycler
Automated Thermal Cycler (ATC)

Which thermocyclers can be controlled using the Thermal Cycler Fleet Control Software?

The Thermal Cycler Fleet Control Software is compatible with the following instruments:
- SimpliAmp Thermal Cycler
- ProFlex PCR System
- Veriti Thermal Cycler
- VeritiPro Thermal Cycler
- Automated Thermal Cycler (ATC)
- MiniAmp Thermal Cycler
- MiniAmp Plus Thermal Cycler

Is there wireless connectivity with the Proflex PCR System?

The ProFlex PCR System can be connected wirelessly with an optional High-Power USB Wi-Fi Module, Cat. No. A26774. Please note that you will need to update to the latest version of firmware in order to use this Wi-Fi module.

How can I get additional throughput for the Applied Biosystems QuantStudio 12K Flex OpenArray System?

Additional Applied Biosystems OpenArray plates can be run off-line using a flat-block thermal cycler. The two thermal cyclers we recommend are the Applied Biosystems GeneAmp PCR System 9700 and the Applied Biosystems ProFlex PCR System.

For Research Use Only. Not for use in diagnostic procedures.