Invitrogen™ Progesterone Metabolites ELISA Kit

Description

ELISA

A progesterone metabolites standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit progesterone metabolites antibodies and progesterone metabolites-peroxidase conjugate is added to the wells. The progesterone metabolites-peroxidase conjugate and any progesterone metabolites in the sample will compete to bind to the anti-rabbit progesterone metabolites antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound progesterone metabolites-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of progesterone metabolites present in the sample.

Progesterone, C21H30O2, also known as P4 (pregn-4-ene-3,20-dione) is a C-21 steroid hormone involved in the female menstrual cycle, gestation and embryogenesis of humans and other species. Progesterone belongs to a class of hormones called progestogens and is the major naturally occurring human progestogen. Progesterone is an essential regulator of human female reproductive function in the uterus, ovary, mammary gland and brain, and plays an important role in non-reproductive tissues such as the cardiovascular system, bone and the central nervous system.

Specifications

• Accession Number: C21H30O2; Progesterone Metabolites; progestogen metabolites
• Assay Range: 102.4 to 10,000 pg/mL
• Assay Sensitivity: 51.2 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Fecal Extract, Plasma, Serum, Supernatant, Urine
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 7%
• Intraassay CV: 7
• Kit Contents: Anti-IgG coated 96 well plate, Standard, Assay Buffer Concentrate, Capture Antibody, HRP-conjugated Antigen, Wash Buffer Concentrate, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 12.5 μL
• Storage Requirements: -20°C
• Target: Progesterone Metabolites
• Test Time: 45 mins
• Total Assay Time: 2 hrs 30 mins