Invitrogen™ Human Progesterone Receptor, Ligand Binding Domain, GST Tag Recombinant Protein

Catalog No.	Quantity
A15672	600 μg

Catalog No. A15672 Supplier Invitrogen™ Supplier No. A15672

Description

Recombinant Protein

Progesterone receptor ligand binding domain (PR-LBD) is a 59.83 kDa recombinant human protein (amino acids 675-933) expressed as a glutathione-S-transferase (GST) fusion protein in baculovirus-infected insect cells. PR is expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies. PR-LBD human recombinant protein is: Flexible-use in your in-house assay or as part of our PolarScreen™ Competitor assays for a complete assay solution Ready to use-active and tagged (with GST) Convenient-available in bulk sizes Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond. Used in:PolarScreen™ Progesterone Receptor Competitor Assay, RedPolarScreen™ Progesterone Receptor Competitor Assay, Green Protein Form: Ligand Binding Domain, Recombinant.

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

Specifications

• Accession Number: P06401
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: Proprietary buffer with no preservative
• Gene ID (Entrez): 5241
• Name: Human Progesterone Receptor, Ligand Binding Domain, GST Tag
• Purification Method: Purified
• Quantity: 600 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 9930019P03Rik; BB114106; ENSMUSG00000074510; LOW QUALITY PROTEIN: progesterone receptor; NR3C3; Nuclear receptor subfamily 3 group C member 3; p4 receptor; PGR; pot. alt. progesterone receptor; putative; PR; PR-A; PR-B; Progesterone receptor; progesterone receptor A; progesterone-receptor; putative form B; steroid hormone receptor; testicular progesterone receptor; unnamed protein product
• Common Name: Progesterone Receptor
• Gene Symbol: PGR
• Protein Length: 675-933
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 675-933
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: Baculovirus
• Protein Family: Growth Factors & Receptors
• Protein Form: Ligand Binding Domain, Recombinant
• Form: Liquid