Invitrogen™ Human Prolactin ELISA Kit

Description

ELISA

The Human Prolactin ELISA quantitates human prolactin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Prolactin. Principle of the method The Human Prolactin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor.

Specifications

• Accession Number: P01236
• Assay Range: 15.6 to 1,000 pg/mL
• Assay Sensitivity: 11.7 pg/mL
• Description: Human Prolactin ELISA Kit
• Assay: Multiplex assay on the Luminex Platform
• Conjugate: HRP
• Detection Method: Fluorescence
• Product Type: ELISA
• Protein Family: Peptide hormone
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5617
• Gene Symbol: PRL
• Interassay CV: 10.8%
• Intraassay CV: 7.6
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Reproductive Biology
• Sample Volume: Plasma 12.5 μL, Serum 12.5 μL, Supernatant 50 μL
• Storage Requirements: -20°C
• Target: Prolactin
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 30 mins