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Invitrogen™ Human Prolactin ELISA Kit

Catalog No. EHIAPRL
Encompass_Preferred
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests

ELISA

The Human Prolactin ELISA quantitates human prolactin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Prolactin. Principle of the method The Human Prolactin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01236
Assay Range 15.6 to 1,000 pg/mL
Assay Sensitivity 11.7 pg/mL
Description Human Prolactin ELISA Kit
Assay Multiplex assay on the Luminex Platform
Conjugate HRP
Detection Method Fluorescence
Product Type ELISA
Protein Family Peptide hormone
Sample Type Plasma, Serum, Supernatant
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 5617
Gene Symbol PRL
Interassay CV 10.8%
Intraassay CV 7.6
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Reproductive Biology
Sample Volume Plasma 12.5 μL, Serum 12.5 μL, Supernatant 50 μL
Storage Requirements -20°C
Target Prolactin
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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