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Promega cAMP-Glo™ Max Assay System

Catalog No. PRV1683 Shop All Promega Corporation Products
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Quantity:
10 x 20 Plates

Description

Includes

  • Protein Kinase A (V135A) : 1 x 20μL
  • cAMP-Glo™ ONE Buffer (V166A) : 1 x 2.5mL
  • MgCl2, 1M (V330A) : 1 x 1mL
  • cAMP, 1mM (V642B) : 1 x 100μL
  • Kinase-Glo™ Substrate (V672A) : 1 x 1 vial
  • Kinase-Glo™ Buffer (V673A) : 1 x 10mL

The cAMP-Glo™ Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate GPCRs coupled with adenylate cyclase typically alter intracellular cAMP levels.

  • Faster cAMP Detection for High-Throughput Assays
  • Complete in ∼30 minutes
  • Scalable to 1536-well plate formats and beyond
  • No interference by fluorescent compounds

Specifications

Includes 1 x 20μL Protein Kinase A, 1 x 2.5mL cAMP-Glo™ one Buffer, 1 x 1mL 1M MgCl2, 1mM 1 x 100μL cAMP, Kinase-Glo™ substrate 1 x 1 Vial, 1 x 10mL Kinase-Glo™ Buffer
pH 7.5
Content And Storage -30°C to -10°C
For Use With (Application) High-throughput analysis of library compounds to identify modulators of GPCR activity, easy quantitation of cAMP levels in broad range of cell types
Quantity 10 x 20 Plates
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