Promega Flexi™ Rabbit Reticulocyte Lysate System

Flexi Rabbit Reticulocyte Lysate System allows optimization of translation reactions for a range of parameters, including Mg(2+) and K(+) and DTT concentration.

Manufacturer:  Promega L4540

Catalog No. PR-L4540

Includes: Luciferase Control RNA; Luciferase Assay Wells; Luciferase Assay Reagent; DTT; Flexi Rabbit Reticulocyte Lysate; Amino acid mixtures; Magnesium acetate; Potassium chloride

Description

• Optimized Translation for Greater Protein Production
• Increase protein production as much as fourfold over standard lysate reactions
• For easy optimization the endogenous magnesium concentration is provided for each lot of Flexi(R) Lysate
• The Flexi(R) System contains three Amino Acid Mixtures, providing a choice of radioisotopes

U.S. Patents 5,641,641; 5,650,289; and 5,814,471; Australian Patent 649289 and other patents and patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
Australian Patents 616881 and 641261 and other pending and issued patents, which claim vectors encoding a portion of human placental ribonuclease inhibitor, are exclusively licensed to Promega Corporation.

Specifications

• For Use With (Application): Optimizing translation of low-expressing messages, oxidizing environment can be adjusted, Luciferase activity can be used for the detection of inhibitory contaminants in RNA preparations, investigation of translational control, screening of chemical libraries for effect on translation
• Includes: Luciferase control RNA, Luciferase assay wells, Luciferase assay reagent, DTT, Flexi Rabbit reticulocyte lysate, Amino Acid mixtures, Magnesium Acetate, Potassium Chloride
• Quantity: 30 x 50μL Reactions
• Storage Requirements: <−65°C