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Promega E. coli S30 Extract System for Linear Templates

The E. coli S30 Extract System for Linear Templates allows transcription/translation of linear DNA templates.

Manufacturer:  Promega L1030

Catalog No. PR-L1030

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Includes: S30 Extract, Linear, 3 × 150μL; Luciferase Dilution Reagent, 1mL; pBESTluc™ DNA, Linear (Control), 10μg; Luciferase Assay Reagent, 250μL; S30 Premix without amino acids, 750μL; Amino acid mixtures, 3 × 175μL



  • Successful Transcription/Translation of Linear DNA Templates

U.S. Patents 5,641,641; 5,650,289; and 5,814,471; Australian Patent 649289 and other patents and patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.



Identification of cloned or synthesized genes from linear DNA, rapid structure mapping of genes without additional cloning steps, rapid verification of in-vitro-generated mutations, synthesis of truncated gene products for functional domain mapping and epitope mapping studies, expression from in-vitro-generated RNA for genes lacking an appropriate E. coli promoter
3 x 150μL S30 linear extract, 1mL Luciferase Dilution Reagent, 10μg pBESTluc™ DNA, Linear (control), 250μL Luciferase Assay Reagent, 750μL S30 premix without Amino A
30 Reactions


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