Promega E. coli T7 S30 Extract System for Circular DNA

Description

Includes

PinPoint™ Xa Control Vector, 5μg; T7/S30 extract for circular DNA, 3 x 150μL; S30 premix without amino acids, 750μL; Amino acid mixture minus methionine, 175μL; Amino acid mixture minus cysteine, 175μL; Amino acid mixture minus leucine, 175μL

The E. coli T7 S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors containing a T7 promoter.

• Create Radiolabeled Protein for Transcription/Translation Studies
• Translate using clones with a T7 promoter and a ribosome binding site; no E. coli promoter required
• Reduce the chance of expressed proteins degrading
• Includes all components needed for coupled transcription/translation

For research purposes only. Not for diagnostic or therapeutic use. For nonresearch uses of the portion of the vector encoding the biotinylation sequence, please contact Promega Corporation for licensing information.

Specifications

• Content And Storage: <-65°C
• For Use With (Application): Synthesis of radiolabeled Protein, verification of cloned gene expression before in-vivo Protein expression in E. coli, use of Protein in functional studies of transcription and translation, use of Protein as a tracer in Protein purification, incorporation of un-natural Amino Acids into Proteins for structural studies
• Includes: 5μg PinPoint Xa control vector, 3 x 150μL T7/S30 extract for circular DNA, 750μL S30 premix without Amino Acids, 175μL Amino Acid mixture minus Methionine, 175μL Ami
• No. of Reactions: 30
• Quantity: 30 Reactions