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Description
- Reverse transcribes RNA templates from total RNA, poly(A)+ mRNA, or synthetic transcript RNA
- Enables robust, full-length cDNA synthesis for the analysis of rare or long messages (up to 8.9kb)
- Uses volumes of 1–20μL from the initial RT reaction in coupled or uncoupled PCR reactions
- May also be used for the incorporation of regular, Cy™3-modified, Cy5-modified, and amino-allyl-modified nucleotides for microarray applications
- Refill components available separately
Specifications
Specifications
| Content And Storage | -30°C to -10°C |
| For Use With (Application) | Reverse transcription of RNA templates from total RNA, poly(A)+ mRNA, or synthetic transcript RNA |
| Includes | Upstream control primer 100μL, Downstream control primer 100μL, MgCl2 1.2mL, Oligo(dT)15 Primer 50μg, dNTP mix 320μL, Random primers 50μg, 1.2kb kanamycin positive control RNA 5μg, ImProm-II 5X reaction buffer 600μL, ImProm-II reverse transcriptase 100μL, Recombinant RNasin ribonuclease inhibitor 2500U, Nuclease-free water 2 × 1250μL |
| No. of Reactions | 100 |
| pH | 7 |
| Product Type | ImProm-II Reverse Transcription System |
| Form | Liquid |
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