Promega ImProm-II™ Reverse Transcription

Description

Formulated for stand-alone applications or for easy transition to gene-specific target amplification.

• Reverse transcribes RNA templates from total RNA, poly(A)+ mRNA, or synthetic transcript RNA
• Enables robust, full-length cDNA synthesis for the analysis of rare or long messages (up to 8.9kb)
• Uses volumes of 1–20μL from the initial RT reaction in coupled or uncoupled PCR reactions
• May also be used for the incorporation of regular, Cy™3-modified, Cy5-modified, and amino-allyl-modified nucleotides for microarray applications
• Refill components available separately

Specifications

• Content And Storage: -30°C to -10°C
• For Use With (Application): Reverse transcription of RNA templates from total RNA, poly(A)+ mRNA, or synthetic transcript RNA
• Includes: Upstream control primer 100μL, Downstream control primer 100μL, MgCl₂ 1.2mL, Oligo(dT)₁₅ Primer 50μg, dNTP mix 320μL, Random primers 50μg, 1.2kb kanamycin positive control RNA 5μg, ImProm-II 5X reaction buffer 600μL, ImProm-II reverse transcriptase 100μL, Recombinant RNasin ribonuclease inhibitor 2500U, Nuclease-free water 2 × 1250μL
• No. of Reactions: 100
• pH: 7
• Product Type: ImProm-II Reverse Transcription System
• Form: Liquid