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Promega LigaFast™ Rapid DNA Ligation System

Catalog No. p-127589 Shop All Promega Corporation Products
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Product Type:
DNA Ligation System
Ligation Buffer
Quantity:
1.5 mL
150 Reactions
30 Reactions
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Catalog No. Product Type Quantity
PR-C6711 Ligation Buffer 1.5 mL
PR-M8221 DNA Ligation System 30 Reactions
PR-M8225 DNA Ligation System 150 Reactions
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Catalog No. PR-C6711 Supplier Promega Supplier No. C6711
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Description

Designed to eliminate the need for further purification prior to transformation of ligated DNA

  • Use with 5', 3' or blunt-ended DNA inserts
  • Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature
  • No requirement to purify ligated DNA prior to heat-shock transformation in E. coli ligations conducted at room temperature
  • No additional buffer modifications required prior to use
  • Ligations performed using the LigaFast™ system are comparable to standard overnight ligations
  • Blue/White Cloning Qualified

Specifications

Content And Storage -30°C to -10°C
Formulation Tris HCl (pH 7) 10mM, KCl 50mM, DTT 1mM, EDTA 0.1mM, Glycerol 50%
For Use With (Application) 5′, 3′ or blunt ended DNA inserts
pH 7.8
Product Type Ligation Buffer
Quantity 1.5 mL
Form Fluid
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