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Promega MagneHis™ Protein Purification System

The MagneHis Protein Purification System provides a magnetic-based, rapid method for the purification of polyhistidine- or HQ-tagged, expressed proteins.

Manufacturer:  Promega V8500

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Catalog No. PR-V8500

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Includes: MagneHis binding/wash buffer, 50mL; FastBreak™ cell lysis reagent, 15mL; DNase I, lyophilized, 1 vial; MagneHis elution buffer, 10mL; MagneHis Ni-particles, 2mL



  • Magnetic Purification of Polyhistidine- or HQ-tagged Proteins from E. coli or Mammalian Cell Lysates
  • No centrifugation or vacuum required after cell lysis
  • Particles are compatible with a variety of common buffers
  • Binding capacity up to 1mg of polyhistidine-tagged protein per 1mL of particles

U.S. Pat. Nos. 7,112,552; 7,304,015; 7,304,016; 7,354,750; 6,174,704; and 7,319,021 and other patents and patents pending.
Certain applications of this product are covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.



50mL MagneHis binding/Wash Buffer, 15mL FastBreak™ cell lysis reagent, DNase I, 1 vial lyophilized, 10mL MagneHis Elution Buffer, 2mL MagneHis Ni-particles
65 Reactions
Protein Purification System
2°C to 10°C
Rapid and reliable purification of Polyhistidine-tagged Proteins from 1mL to 1L of bacterial culture


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