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Promega NanoLuc™ pNL1 Vectors

Configured with NanoLuc Luciferase for use in reporter gene assays of transcriptional control—ideal for cloning of known or putative promoter region

Manufacturer:  Promega N1101

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Catalog No. PRN1101


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Description

Description

Multiple forms of luciferase have been configured to meet differing experimental objectives. Unfused Nluc offers maximal light output and sensitivity, NanoLuc-PEST (NlucP) closely couples protein expression to changes in transcriptional activity and increased signal-to background ratios, and NanoLuc luciferase fused to an N-terminal secretion signal (secNluc) is suitable when a secreted reporter is preferred.

NanoLuc (Nluc) Luciferase Technology:

  • NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter
  • Luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity
  • Luminescence is linearly proportional to amount of NanoLuc protein over 1,000,000-fold concentration range
  • Signal half-life ≥2 hours when detected with Nano-Glo™ Luciferase Assay Reagent
Nanoluc Luciferase Enzyme:
  • Monomeric, with 171 amino acids and 513 base pairs
  • With high thermal stability and broad pH range
  • No post-translational modifications or disulfide bonds
  • Uniform distribution in cells
  • Emission spectrum well suited for bioluminescence resonance energy transfer
Nluc Vector:
  • Uses pGL4-based backbone for easy sequence transfer from existing plasmids
  • Reduces anomalous results by removing many transcription factor binding sites and other potential regulatory elements
  • Nluc gene variations are codon optimized and have many potential regulatory elements or other undesirable features removed, such as common restriction enzyme sites

Transcription regulation, Virus-cell interactions, Compound screening, Post-translational modifications, GPCR signaling, Cell signaling, Promoter analysis

Specifications

Specifications

pNL1.3.CMV(secNluc/CMV)
8
−30 to −10°C
Liquid
20μg
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