Promega pGL4 Luciferase Reporter Vectors

Available in numerous configurations for enhanced usability and convenience

Catalog Number	Mfr. No.	Reporter Gene	Storage Buffer	Vector
PRE6651	Promega™ E6651	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.10[luc2] vector
PRE6661	Promega™ E6661	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.11[luc2P] vector
PRE6681	Promega™ E6681	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.13[luc2/SV40] vector
PRE6721	Promega™ E6721	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.17[luc2/Neo] vector
PRE6671	Promega Corporation E6671	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.12[luc2CP] vector
PRE6731	Promega™ E6731	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.18[luc2P/Neo] vector
PRE6911	Promega™ E6911	hRlucB	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.73[hRluc/SV40] vector
PRE6881	Promega™ E6881	hRlucB	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.70[hRluc] vector
PRE6921	Promega™ E6921	hRlucB	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.74[hRluc/TK] vector
PRE6931	Promega™ E6931	hRlucB	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.75[hRluc/CMV] vector
PRE7521	Promega™ E7521	hRlucB	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.84[hRlucCP/Puro] vector
PRE6751	Promega™ E6751	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.20[luc2/Puro] vector
PRE6701	Promega™ E6701	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.15[luc2P/Hygro] vector
PRE6691	Promega™ E6691	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.14[luc2/Hygro] vector
PRE8491	Promega™ E8491	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.32[luc2P/NF-κB-RE/Hygro] vector
PRE1340	Promega™ E1340	luc2A	10mM Tris HCl, 1mM EDTA (pH 8)	pGL4.33[luc2P/SRE/Hygro] vector
PRE1350	Promega™ E1350	luc2A	10mM Tris HCl, 1mM EDTA (pH 8)	pGL4.34[luc2P/SRF-RE/Hygro] vector
PRE1360	Promega™ E1360	luc2A	10mM Tris HCl, 1mM EDTA (pH 8)	pGL4.36[luc2P/MMTV/Hygro] vector
PRE1370	Promega™ E1370	luc2A	10mM Tris HCl, 1mM EDTA (pH 7.4 at 25°C)	pGL4.35[luc2P/9XGAL4UAS/Hygro] vector
PRC9351	Promega™ C9351	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.31[luc2P/GAL4UAS/Hygro] vector
PRE1310	Promega™ E1310	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.50[ luc2 /CMV/Hygro] vector
PRE1320	Promega™ E1320	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.51[luc2/CMV/Neo] vector
PRE8411	Promega™ E8411	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.23[luc2/minP] vector
PRE8431	Promega™ E8431	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.25[luc2CP/minP] vector
PRE8441	Promega™ E8441	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.26[luc2/minP/Hygro] vector
PRE8421	Promega™ E8421	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.24[luc2P/minP] vector
PRE8451	Promega™ E8451	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.27[luc2P/minP/Hygro] vector
PRE8481	Promega™ E8481	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.30[luc2P/NFAT-RE/Hygro] vector
PRE8461	Promega™ E8461	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.28[luc2CP/minP/Hygro] vector
PRE8471	Promega™ E8471	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.29[luc2P/CRE/Hygro] vector
PRE3641	Promega™ E3641	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.37[luc2P/ARE/Hygro] vector
PRE4001	Promega™ E4001	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.42[luc2P/HRE/Hygro] vector
PRE4121	Promega™ E4121	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.43[luc2P/XRE/Hygro] vector
PRE4111	Promega™ E4111	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.44[luc2P/AP1 RE/Hygro] vector
PRE4651	Promega™ E4651	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.52[luc2P/STAT5RE/Hygro] vector
PRE6951	Promega™ E6951	hRlucB	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.77[hRlucP/Hygro] vector

Description

• Configurations available with the synthetic firefly luc2 (Photinus pyralis) and Renilla hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells
• Both the reporter genes and the vector backbone, including the bla (β-lactamase or Amp^r) and mammalian selectable marker genes for hygromycin (Hygro or Hyg^r), neomycin (Neo or Neo^r) and puromycin (Puro or Puro^r), have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription
• The backbone is also supplied with two Rapid Response™ Luciferase Reporter genes (-P, -CP) for each luciferase gene; the proteins encoded by these Rapid Response genes respond more quickly and with greater magnitude to changes in transcriptional activity than their more stable counterparts
• Choice of mammalian selectable markers provides easy transition from transient to stable cells
• Use of a common multiple cloning site and a unique Sfil transfer scheme permits easy transfer from vector to vector

Vector Options

• Basic vectors with no promoter that contain a multiple cloning region for cloning a promoter of choice
• Vectors containing a minimal promoter
• Vectors containing response elements (cAMP, NFAT, NF-κB, GAL4UAS) and a minimal promoter
• Promoter-containing vectors that can be used as expression controls or as coreporter vectors

Transcription regulation, Virus-cell interactions, Compound screening, Post-translational modifications, GPCR signaling, Cell signaling, Promoter analysis

For research use only. This product and/or its use is subject to one or more of the following Promega patents: U.S. Pat. Appln. Ser. Nos. 09/645,706; 10/664,341; 10/943,508; U.S. Patents 5,670,356; 6,387,675 and 6,552,179, Australian Patent 698424 and various corresponding patent applications and issued patents. The terms of the limited license conveyed with the purchase of this product are as follows: Researchers may use this product in their own research and they may transfer derivatives to others for such research use provided that at the time of transfer a copy of this label license is given to the recipients and the recipients agree to be bound by the conditions of this label license. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that Researchers may: (1) clone heterologous DNA sequences at either or both ends of said luciferase gene so as to create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus when compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in furtherance of splicing research predicated on the inactivation or reconstitution of the luminescent activity of the encoded luciferase. In addition, Researchers must do one of the following: (1) use luminescent assay reagents purchased from Promega Corporation for all determinations of luminescence activity resulting from the research use of this product and its derivatives; or (2) contact Promega to obtain a license for the use of the product and its derivatives. No other use or transfer of this product or its derivatives is authorized without the express written consent of Promega including, without limitation, Commercial Use. Commercial Use means any and all uses of this product and derivatives by a party for monetary or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to such Commercial Use, or any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. If the purchaser is not willing to accept the conditions of this limited use statement, Promega is willing to accept the return of the unopened product and provide the purchaser with a full refund. However, in the event the product is opened, then the purchaser agrees to be bound by the conditions of this limited use statement.
Mfr. No. C9351, E1310, E1320, E1370, E6651, E6661, E6671, E6681, E6691, E6701, E6711, E6721, E6731, E6741, E6751, E6761, E6771, E8411, E8421, E8431, E8441, E8451, E8461, E8471, E8481, E8491: The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
Mfr. No. E6881, E6891, E6901, E6911, E6921, E6931, E6941, E6951, E6961, E6971, E6981, E6991, E7501, E7511, E7521: Licensed from University of Georgia Research Foundation, Inc., under U.S. Patent 5,418,155; Canadian Patents 2,105,984 and related patents.
Mfr. No. E8481: The NFAT response element and its use are licensed under one or more of the following patents: U.S. Patents 5,837,840; 6,197,925; 5,989,810; 6,096,515; 6,388,052; 6,150,099; 6,171,781; 6,352,830; 6,312,899 and 6,875,571.
Mfr. No. E1310, E1320: The use of this product and derivatives thereof is strictly limited to that of a life sciences research reagent. All other use is strictly prohibited, including but not limited to any diagnostic, therapeutic, or commercial use. The use of this product for in vivo bioluminescent imaging may be covered by one or more patents controlled by Caliper Life Sciences, Inc. Those patents include U.S. Patents 5,650,135; 6,217,847; 6,923,951; 6,908,605; 6,890,515; and corresponding foreign patents and pending applications. The purchase or transfer of this product is not intended, either expressly or by implication, to grant any right or license to practice under the foregoing patents. Purchasers are advised to contact Caliper Life Sciences, Inc., 68 Elm St., Hopkinton, MA 01748 for information regarding a license to practice under such patents.

Specifications

• Concentration: 1μg/μL
• For Use With (Application): Transcription regulation, virus-cell interactions, compound screening, post-translational modifications, GPCR signaling, cell signaling, promoter analysis
• Quantity: 20μg
• Storage Requirements: −30 to −10°C