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Promega pGL4 Luciferase Reporter Vectors
Available in numerous configurations for enhanced usability and convenience
$514.00 - $539.00
Specifications
Concentration | 1μg/μL |
---|---|
For Use With (Application) | Transcription regulation, virus-cell interactions, compound screening, post-translational modifications, GPCR signaling, cell signaling, promoter analysis |
Quantity | 20μg |
Storage Requirements | −30 to −10°C |
Catalog Number | Mfr. No. | Reporter Gene | Storage Buffer | Vector | Price | Quantity & Availability | |||
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Catalog Number | Mfr. No. | Reporter Gene | Storage Buffer | Vector | Price | Quantity & Availability | |||
PRE6651
|
Promega
E6651 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.10[luc2] vector |
Each for $539.00
|
|
PRE6661
|
Promega
E6661 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.11[luc2P] vector |
Each for $539.00
|
|
PRE6681
|
Promega
E6681 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.13[luc2/SV40] vector |
Each for $539.00
|
|
PRE6721
|
Promega
E6721 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.17[luc2/Neo] vector |
Each for $539.00
|
|
PRE6671
|
Promega Corporation
E6671 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.12[luc2CP] vector |
Each for $539.00
|
|
PRE6731
|
Promega
E6731 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.18[luc2P/Neo] vector |
Each for $539.00
|
|
PRE6911
|
Promega
E6911 |
hRlucB | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.73[hRluc/SV40] vector |
Each for $539.00
|
|
PRE6881
|
Promega
E6881 |
hRlucB | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.70[hRluc] vector |
Each for $539.00
|
|
PRE6921
|
Promega
E6921 |
hRlucB | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.74[hRluc/TK] vector |
Each for $539.00
|
|
PRE6931
|
Promega
E6931 |
hRlucB | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.75[hRluc/CMV] vector |
Each for $539.00
|
|
PRE7521
|
Promega
E7521 |
hRlucB | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.84[hRlucCP/Puro] vector |
Each for $539.00
|
|
PRE6751
|
Promega
E6751 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.20[luc2/Puro] vector |
Each for $539.00
|
|
PRE6701
|
Promega
E6701 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.15[luc2P/Hygro] vector |
Each for $539.00
|
|
PRE6691
|
Promega
E6691 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.14[luc2/Hygro] vector |
Each for $539.00
|
|
PRE8491
|
Promega
E8491 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.32[luc2P/NF-κB-RE/Hygro] vector |
Each for $539.00
|
|
PRE1340
|
Promega
E1340 |
luc2A | 10mM Tris HCl, 1mM EDTA (pH 8) | pGL4.33[luc2P/SRE/Hygro] vector |
Each for $539.00
|
|
PRE1350
|
Promega
E1350 |
luc2A | 10mM Tris HCl, 1mM EDTA (pH 8) | pGL4.34[luc2P/SRF-RE/Hygro] vector |
Each for $539.00
|
|
PRE1360
|
Promega
E1360 |
luc2A | 10mM Tris HCl, 1mM EDTA (pH 8) | pGL4.36[luc2P/MMTV/Hygro] vector |
Each for $539.00
|
|
PRE1370
|
Promega
E1370 |
luc2A | 10mM Tris HCl, 1mM EDTA (pH 7.4 at 25°C) | pGL4.35[luc2P/9XGAL4UAS/Hygro] vector |
Each for $539.00
|
|
PRC9351
|
Promega
C9351 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.31[luc2P/GAL4UAS/Hygro] vector |
Each for $514.00
|
|
PRE1310
|
Promega
E1310 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.50[ luc2 /CMV/Hygro] vector |
Each for $539.00
|
|
PRE1320
|
Promega
E1320 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.51[luc2/CMV/Neo] vector |
Each for $539.00
|
|
PRE8411
|
Promega
E8411 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.23[luc2/minP] vector |
Each for $539.00
|
|
PRE8431
|
Promega
E8431 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.25[luc2CP/minP] vector |
Each for $539.00
|
|
PRE8441
|
Promega
E8441 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.26[luc2/minP/Hygro] vector |
Each for $539.00
|
|
PRE8421
|
Promega
E8421 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.24[luc2P/minP] vector |
Each for $539.00
|
|
PRE8451
|
Promega
E8451 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.27[luc2P/minP/Hygro] vector |
Each for $539.00
|
|
PRE8481
|
Promega
E8481 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.30[luc2P/NFAT-RE/Hygro] vector |
Each for $539.00
|
|
PRE8461
|
Promega
E8461 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.28[luc2CP/minP/Hygro] vector |
Each for $539.00
|
|
PRE8471
|
Promega
E8471 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.29[luc2P/CRE/Hygro] vector |
Each for $539.00
|
|
PRE3641
|
Promega
E3641 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.37[luc2P/ARE/Hygro] vector |
Each for $539.00
|
|
PRE4001
|
Promega
E4001 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.42[luc2P/HRE/Hygro] vector |
Each for $539.00
|
|
PRE4121
|
Promega
E4121 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.43[luc2P/XRE/Hygro] vector |
Each for $539.00
|
|
PRE4111
|
Promega
E4111 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.44[luc2P/AP1 RE/Hygro] vector |
Each for $539.00
|
|
PRE4651
|
Promega
E4651 |
luc2A | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.52[luc2P/STAT5RE/Hygro] vector |
Each for $539.00
|
|
PRE6951
|
Promega
E6951 |
hRlucB | 10mM Tris HCl (pH 7.4), 1mM EDTA | pGL4.77[hRlucP/Hygro] vector |
This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000. |
N/A |
Description
- Configurations available with the synthetic firefly luc2 (Photinus pyralis) and Renilla hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells
- Both the reporter genes and the vector backbone, including the bla (β-lactamase or Ampr) and mammalian selectable marker genes for hygromycin (Hygro or Hygr), neomycin (Neo or Neor) and puromycin (Puro or Puror), have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription
- The backbone is also supplied with two Rapid Response Luciferase Reporter genes (-P, -CP) for each luciferase gene; the proteins encoded by these Rapid Response genes respond more quickly and with greater magnitude to changes in transcriptional activity than their more stable counterparts
- Choice of mammalian selectable markers provides easy transition from transient to stable cells
- Use of a common multiple cloning site and a unique Sfil transfer scheme permits easy transfer from vector to vector
Vector Options
- Basic vectors with no promoter that contain a multiple cloning region for cloning a promoter of choice
- Vectors containing a minimal promoter
- Vectors containing response elements (cAMP, NFAT, NF-κB, GAL4UAS) and a minimal promoter
- Promoter-containing vectors that can be used as expression controls or as coreporter vectors
Transcription regulation, Virus-cell interactions, Compound screening, Post-translational modifications, GPCR signaling, Cell signaling, Promoter analysis
For research use only. This product and/or its use is subject to one or more of the following Promega patents: U.S. Pat. Appln. Ser. Nos. 09/645,706; 10/664,341; 10/943,508; U.S. Patents 5,670,356; 6,387,675 and 6,552,179, Australian Patent 698424 and various corresponding patent applications and issued patents. The terms of the limited license conveyed with the purchase of this product are as follows: Researchers may use this product in their own research and they may transfer derivatives to others for such research use provided that at the time of transfer a copy of this label license is given to the recipients and the recipients agree to be bound by the conditions of this label license. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that Researchers may: (1) clone heterologous DNA sequences at either or both ends of said luciferase gene so as to create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus when compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in furtherance of splicing research predicated on the inactivation or reconstitution of the luminescent activity of the encoded luciferase. In addition, Researchers must do one of the following: (1) use luminescent assay reagents purchased from Promega Corporation for all determinations of luminescence activity resulting from the research use of this product and its derivatives; or (2) contact Promega to obtain a license for the use of the product and its derivatives. No other use or transfer of this product or its derivatives is authorized without the express written consent of Promega including, without limitation, Commercial Use. Commercial Use means any and all uses of this product and derivatives by a party for monetary or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to such Commercial Use, or any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. If the purchaser is not willing to accept the conditions of this limited use statement, Promega is willing to accept the return of the unopened product and provide the purchaser with a full refund. However, in the event the product is opened, then the purchaser agrees to be bound by the conditions of this limited use statement.
Mfr. No. C9351, E1310, E1320, E1370, E6651, E6661, E6671, E6681, E6691, E6701, E6711, E6721, E6731, E6741, E6751, E6761, E6771, E8411, E8421, E8431, E8441, E8451, E8461, E8471, E8481, E8491: The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
Mfr. No. E6881, E6891, E6901, E6911, E6921, E6931, E6941, E6951, E6961, E6971, E6981, E6991, E7501, E7511, E7521: Licensed from University of Georgia Research Foundation, Inc., under U.S. Patent 5,418,155; Canadian Patents 2,105,984 and related patents.
Mfr. No. E8481: The NFAT response element and its use are licensed under one or more of the following patents: U.S. Patents 5,837,840; 6,197,925; 5,989,810; 6,096,515; 6,388,052; 6,150,099; 6,171,781; 6,352,830; 6,312,899 and 6,875,571.
Mfr. No. E1310, E1320: The use of this product and derivatives thereof is strictly limited to that of a life sciences research reagent. All other use is strictly prohibited, including but not limited to any diagnostic, therapeutic, or commercial use. The use of this product for in vivo bioluminescent imaging may be covered by one or more patents controlled by Caliper Life Sciences, Inc. Those patents include U.S. Patents 5,650,135; 6,217,847; 6,923,951; 6,908,605; 6,890,515; and corresponding foreign patents and pending applications. The purchase or transfer of this product is not intended, either expressly or by implication, to grant any right or license to practice under the foregoing patents. Purchasers are advised to contact Caliper Life Sciences, Inc., 68 Elm St., Hopkinton, MA 01748 for information regarding a license to practice under such patents.
Specifications
1μg/μL | |
Transcription regulation, virus-cell interactions, compound screening, post-translational modifications, GPCR signaling, cell signaling, promoter analysis | |
20μg | |
−30 to −10°C |
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