Promega pGL4 Luciferase Reporter Vectors

Available in numerous configurations for enhanced usability and convenience

Catalog Number	Mfr. No.	Reporter Gene	Storage Buffer	Vector
PRE6651	Promega E6651	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.10[luc2] vector
PRE6661	Promega E6661	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.11[luc2P] vector
PRE6681	Promega E6681	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.13[luc2/SV40] vector
PRE6731	Promega E6731	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.18[luc2P/Neo] vector
PRE6911	Promega E6911	hRlucB	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.73[hRluc/SV40] vector
PRE6881	Promega E6881	hRlucB	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.70[hRluc] vector
PRE6921	Promega E6921	hRlucB	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.74[hRluc/TK] vector
PRE6931	Promega E6931	hRlucB	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.75[hRluc/CMV] vector
PRE7521	Promega E7521	hRlucB	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.84[hRlucCP/Puro] vector
PRE6751	Promega E6751	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.20[luc2/Puro] vector
PRE6701	Promega E6701	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.15[luc2P/Hygro] vector
PRE8491	Promega E8491	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.32[luc2P/NF-κB-RE/Hygro] vector
PRE1340	Promega E1340	luc2A	10mM Tris HCl, 1mM EDTA (pH 8)	pGL4.33[luc2P/SRE/Hygro] vector
PRE1350	Promega E1350	luc2A	10mM Tris HCl, 1mM EDTA (pH 8)	pGL4.34[luc2P/SRF-RE/Hygro] vector
PRE1360	Promega E1360	luc2A	10mM Tris HCl, 1mM EDTA (pH 8)	pGL4.36[luc2P/MMTV/Hygro] vector
PRE1370	Promega E1370	luc2A	10mM Tris HCl, 1mM EDTA (pH 7.4 at 25°C)	pGL4.35[luc2P/9XGAL4UAS/Hygro] vector
PRC9351	Promega C9351	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.31[luc2P/GAL4UAS/Hygro] vector
PRE1310	Promega E1310	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.50[ luc2 /CMV/Hygro] vector
PRE1320	Promega E1320	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.51[luc2/CMV/Neo] vector
PRE8411	Promega E8411	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.23[luc2/minP] vector
PRE8431	Promega E8431	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.25[luc2CP/minP] vector
PRE8441	Promega E8441	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.26[luc2/minP/Hygro] vector
PRE8421	Promega E8421	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.24[luc2P/minP] vector
PRE8451	Promega E8451	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.27[luc2P/minP/Hygro] vector
PRE8481	Promega E8481	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.30[luc2P/NFAT-RE/Hygro] vector
PRE8461	Promega E8461	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.28[luc2CP/minP/Hygro] vector
PRE8471	Promega E8471	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.29[luc2P/CRE/Hygro] vector
PRE3641	Promega E3641	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.37[luc2P/ARE/Hygro] vector
PRE4001	Promega E4001	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.42[luc2P/HRE/Hygro] vector
PRE4121	Promega E4121	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.43[luc2P/XRE/Hygro] vector
PRE4111	Promega E4111	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.44[luc2P/AP1 RE/Hygro] vector
PRE4651	Promega E4651	luc2A	10mM Tris HCl (pH 7.4), 1mM EDTA	pGL4.52[luc2P/STAT5RE/Hygro] vector

Description

• Configurations available with the synthetic firefly luc2 (Photinus pyralis) and Renilla hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells
• Both the reporter genes and the vector backbone, including the bla (β-lactamase or Amp^r) and mammalian selectable marker genes for hygromycin (Hygro or Hyg^r), neomycin (Neo or Neo^r) and puromycin (Puro or Puro^r), have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription
• The backbone is also supplied with two Rapid Response™ Luciferase Reporter genes (-P, -CP) for each luciferase gene; the proteins encoded by these Rapid Response genes respond more quickly and with greater magnitude to changes in transcriptional activity than their more stable counterparts
• Choice of mammalian selectable markers provides easy transition from transient to stable cells
• Use of a common multiple cloning site and a unique Sfil transfer scheme permits easy transfer from vector to vector

Vector Options

• Basic vectors with no promoter that contain a multiple cloning region for cloning a promoter of choice
• Vectors containing a minimal promoter
• Vectors containing response elements (cAMP, NFAT, NF-κB, GAL4UAS) and a minimal promoter
• Promoter-containing vectors that can be used as expression controls or as coreporter vectors

Transcription regulation, Virus-cell interactions, Compound screening, Post-translational modifications, GPCR signaling, Cell signaling, Promoter analysis

Specifications

• Content And Storage: -30°C to -10°C
• Concentration: 1 μg/μL
• Quantity: 20 μg
• For Use With (Application): Transcription regulation, virus-cell interactions, compound screening, post-translational modifications, GPCR signaling, cell signaling, promoter analysis