Promega pmirGLO Dual-Luciferase miRNA Target Expression Vector

Description

Includes

pmirGLO vector, 20μg; Oligo annealing buffer, 1mL

The pmirGlo™ Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3' of the firefly luciferase gene (luc2).

• Evaluate microRNA Activity by Inserting miRNA Target Sites 3′ of the Firefly Luciferase Gene
• Reporter activity correlates with miRNA activity
• Optimized luc2 reporter gene provides highest expression while the Renilla luciferase gene provides normalization
• The moderate-strength PGK promoter provides more biologically relevant analysis not possible with strong promoters

• Limited Use License
• For research use only. The terms of the limited license conveyed with the purchase of this product are as follows: Researchers may use this product in their own research and they may transfer derivatives to others for such research use provided that at the time of transfer a copy of this label license is given to the recipients and the recipients agree to be bound by the conditions of this label license. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that Researchers may: (1) clone heterologous DNA sequences at either or both ends of said luciferase gene so as to create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus when compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in furtherance of splicing research predicated on the inactivation or reconstitution of the luminescent activity of the encoded luciferase. In addition, Researchers must do one of the following: (1) use luminescent assay reagents purchased from Promega Corporation for all determinations of luminescence activity resulting from the research use of this product and its derivatives; or (2) contact Promega to obtain a license for the use of the product and its derivatives. No other use or transfer of this product or its derivatives is authorized without the express written consent of Promega including, without limitation, Commercial Use. Commercial Use means any and all uses of this product and derivatives by a party for monetary or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to such Commercial Use,

Specifications

• Marker Gene: Renilla/Neomycin
• Promoter: PGK
• Reporter Gene: Firefly Luciferase (luc2), Renilla Luciferase (hRluc-neo)
• Storage Buffer: 10mM Tris HCl, 1mM EDTA (pH 7.4)
• Content And Storage: -30°C to -10°C
• Concentration: 1 μg/μL
• Quantity: 20 μg
• Vector: pmirGLO expression vector
• For Use With (Application): miRNA detection, miRNA binding site analysis, transcript stability study, 3'-UTR analysis