Promega SP6 RNA Polymerase

DNA-dependent RNA polymerase for in vitro transcription.

Supplier:  Promega P4084

Catalog No. PRP4084

Description

• DNA-Dependent RNA Polymerase Specific for SP6 Promoter Sequences
• Exhibits extremely high affinity and specificity for SP6 promoter sequences
• >90% pure as determined by SDS polyacrylamide gel electrophoresis
• Free of detectable levels of contaminating RNase and DNase activity (

Specifications

• Concentration: 80 U/μL
• Components: 100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
• pH: 7.5
• Storage Buffer: 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
• Source: Recombinant E. coli strain

• Color: Colorless
• Purity: >90%
• For Use With (Application): Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
• Content And Storage: -30°C to -10°C
• Quantity: 2500 U