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Description
Includes
pGEM™ Express Positive Control Template; RQ1 RNase-free DNase; Nuclease-free Water; T7 Express Enzyme Mix; Sodium Acetate, 3.0M (pH 5.2); RiboMAX Express T7 2X Buffer
- Produces 5-8.5mg/mL RNA in 30 Minutes
- Consistent production of milligram amounts of RNA
- Minimal pipetting steps and setup time
- Efficiently transcribes DNA templates of varying sizes. Works with transcripts as short as 21bp.
Specifications
Specifications
| Content And Storage | -30°C to -10°C |
| For Use With (Application) | Generation of large quantities of uncapped transcripts for in vitro or in vivo translation, in vitro synthesis of tRNA, rRNA, RNA virus genomes and ribozymes, production of substrates for RNA splicing, RNA secondary structure, antisense RNA and RNA:protein interaction studies; RNA for nonmammalian RNAi |
| Includes | 100μL Enzyme Mix, T7 Express; 500μL RiboMAX Express T7 2X Buffer; 110U RQ1 RNase-free DNase; 2 × 5μg pGEM Express Positive Control Template; 1mL Sodium Acetate, 3.0M (pH 5.2); 1250μL Nuclease-free Water |
| Quantity | 50 x 20 μL Reactions |
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