Promega T7 RiboMAX™ Express RNAi System

Description

Includes

T7 Express Enzyme Mix; RiboMAX Express T7 2X Buffer; pGEM Express Positive Control Template; Sodium Acetate, 3.0M (pH 5.2); RNase A Solution; RQ1 RNase-free DNase; Nuclease-free Water

The T7 RiboMAX Express RNAi System is an in vitro transcription system designed to produce milligram amounts of RNA suitable for use in RNAi assays.

• Produces Milligam Amounts of RNA for RNA Interference Experiments
• siRNA duplexes for mammalian RNAi
• Long dsRNA for nonmammalian RNAi
• Produces milligram amounts of double-stranded RNA in 30 minutes

• U.S. Patent 5,552,302.
• This product is covered under license from Carnegie Institution of Washington under U.S. Patent 6,506,559, Australian Patent 743798 and other patents pending. Commercial use of this product will require a separate license from Carnegie.

Specifications

• Content And Storage: -30°C to -10°C
• For Use With (Application): Synthesis of siRNA duplexes for mammalian RNA interference, generation of large quantities of long dsRNA for non-mammalian RNA interference
• Includes: 100μL Enzyme mix, T7 Express (T7 RNA Polymerase, recombinant RNasin™ RNase inhibitor and recombinant inorganic Pyrophosphatase), 500μL RiboMAX™ Express T7 2X Buffer, 110U 1U/
• Quantity: 50 x 20 μL