Promega TNT™ Coupled Reticulocyte Lysate Systems

Description

Includes

TNT Rabbit Reticulocyte Lysate, Appropriate TNT polymerase, Control DNA, TNT Reaction Buffer, Luciferase assay wells, Luciferase assay reagent, Amino acid mixtures

TNT Coupled Reticulocyte Lysate Systems are single-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

• A Single-Tube, Coupled Transcription/Translation System
• Study protein:protein, protein:nucleic acid and protein expression interactions
• Generate proteins in one hour versus days
• You choose the promoter

• U.S. Patents 5,324,637 and 5,492,817; Australian Patent 660329.
• U.S. Patents 5,641,641; 5,650,289; and 5,814,471; Australian Patent 649289.
• Australian Patents 616881 and 641261 and other pending and issued patents, which claim vectors encoding a portion of human placental ribonuclease inhibitor, are exclusively licensed to Promega Corporation.
• The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

Specifications

• Content And Storage: <-65°C
• For Use With (Application): GST pull-downs, Gel shift assays, co-immunoprecipitation, characterization of Protein modifications, Protein-RNA interactions, Protein activity studies, confirmation of gene products, Protein truncation test (PTT)
• Includes: 200μL TNT™ Rabbit Reticulocyte Lysate, 20μL TNT™ Reaction Buffer, 20μL TNT™ T7 or SP6 RNA Polymerase, 5μg Luciferase T7 or SP6 co
• No. of Reactions: 8 Reactions - Trial Size
• Product Type: TnT T7 Coupled Reticulocyte Lysate System