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Promega™ Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay System

Measures the chymotrypsin-like protease activity of the proteasome complex in cultured cells. Add the Proteasome-Glo Cell-Based Assay Reagent in an add-mix-measure format to produce proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.

Manufacturer:  Promega™ G8662

Catalog No. PRG8662


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Description

Description

  • Obtain activity data directly from a cellular environment
  • Add-mix-measure protocol minimizes handling steps and makes the assays amenable to automation
  • Maximum sensitivity is reached 10 to 30 minutes after reagent addition
  • Avoids inherent fluorescent background signals, providing excellent signal-to-background readings
  • Assays are linear over three logs of cell number or four logs of proteasome concentration

'U.S. Pat. No. 6,602,677, Australian Pat. No. 754312 and other patents and patents pending. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673.

Specifications

Specifications

Measuring the chymotrypsin-like activity of the proteasome in cultured cells; screening of inhibitor libraries and monitoring proteasome-regulated protein degradation in cells
2 x 50mL
−30 to −10°C
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