Novus Biologicals™ Protein Disulfide Isomerase/P4HB Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Protein Disulfide Isomerase/P4HB.

Specifications

• Assay Range: 47 pg/mL
• Assay Sensitivity: 47-3000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Protein Disulfide Isomerase/P4HB.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: Cellular thyroid hormone-binding protein, collagen prolyl 4-hydroxylase beta, DSI, ERBA2L, GIT, glutathione-insulin transhydrogenase, P4Hbeta, p55, PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1), PDIEC 5.3.4.1, PHDB, PO4DB, PO4HB, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide, PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55), Prolyl 4-hydroxylase subunit beta, prolyl 4-hydroxylase, beta polypeptide, protein disulfide isomerase family A, member 1, protein disulfide isomerase/oxidoreductase, protein disulfide isomerase-associated 1, protein disulfide-isomerase, protocollagen hydroxylase, thyroid hormone-binding protein p55
• Gene ID (Entrez): 5034
• Gene Symbol: P4HB
• Host Species: Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of mouse anti-Human Protein Disulfide Isomerase/P4HB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of mouse anti-Human Protein Disulfide Isomerase/P4HB monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 85 ng of recombinant Human Protein Disulfide Isomerase/P4HB.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cellular Markers, ER Markers, Fibroblast Cell Markers, Hypoxia
• Storage Requirements: Storage is content dependent.
• Target: Protein Disulfide Isomerase/P4HB
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.