Cytiva Protein G HP MultiTrap™ Plates

Description

Includes

Four prepacked Protein G HP MultiTrap 96-well filter plates with instructions, plus a protocol, with no pretreatment of sample required, for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min.

For small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates

• Flexible format, with protocols for both classic elution and cross-link methods
• Formatted for elution for both electrophoresis and LC-MS analysis workflows
• Fast-binding kinetics and high capacity provide high yield
• Prepacked with Protein G Sepharose™ High Performance Medium for coupling of antibodies of IgG subclasses
• Can be used with centrifugation or vacuum, manually or in automated robotic systems
• Easy scale-up with HiTrap™ Protein G HP prepacked columns

• Matrix: Highly cross-linked agarose, 6%
• Binding capacity: approx. 25mg human IgG/mL medium (0.5 to 1mg IgG/well)
• Average particle size: 34μm
• pH stability: 3 to 9 (long term); 2 to 9 (short term)
• Well volume: 800μL
• Centrifugation force: <700 x G
• Vacuum pressure: -0.5 bar (max.)

Specifications

• Product Type: Protein G Sepharose HP MultiTrap plate
• Quantity: 4 Pack
• Includes: 4 Prepacked Protein G HP MultiTrap 96-well filter plates with instructions, plus a proto col
• Material: Polypropylene, Polyethylene
• Ligand Type: Recombinant Protein G lacking Albumin-binding region (2mg/mL medium)
• Max. Pressure: -0.5 bar
• pH Range: 3 to 9 (long term), 2 to 9 (short term)
• Particle Size: 34μm
• Support Type: 6% Highly Cross-linked Agarose
• Content And Storage: 2° to 8°C
• Column Type: Protein G Sepharose Plate
• For Use With (Application): Small-scale purification of Antibodies or enrichment of Proteins of interest in prepacked 96-well filter plates that are ready to use, electrophoresis or LC/MS