MilliporeSigma™ ProteoExtract™ Native Cytoskeleton Enrichment

Description

Includes

• One bottle containing 1.9mL of 10x Cellular Extraction Buffer
• One bottle containing 3.5mL of 20x Cytoskeleton Wash Buffer
• One bottle containing 9mL of ready to use Nuclear Extraction Buffer
• One vial containing 100μL of 1000x Protease Inhibitor Cocktail solution
• One vial containing 150μL of 1000x Sodium Orthovanadate solution
• One vial containing 50μL of 200x donkey anti-mouse FITC conjugate
• One bottle containing 12.5mL of 10x Blocking/Permeabilization Buffer
• One vial containing 15μg lyophilized TRITC-Conjugated Phalloidin
• One vial containing 100μL of 200x DAPI
• One vial containing 100μL of 100x anti-Vinculin mouse monoclonal IgG1 antibody
• One vial containing 16μL of 500x anti-GAPDH mouse monoclonal IgG1 antibody

Requires

• Pipettors and tips capable of accurately measuring 1-1000μL
• Graduated serological pipettes
• Distilled or deionized water
• 4% paraformaldehyde solution for fixing cells
• 1x Dulbecco's Phosphate Buffered Saline (D-PBS)
• Cover slips
• Mounting fluid

This method provides means to directly study cytoskeleton-associated pool of proteins in normal and diseased cytoskeletons

• Provides cytoskeleton purification detergent buffers, sufficient for extraction from ten 100mm culture dishes to retain focal adhesion and actin-associated proteins while removing soluble cytoplasmic and nuclear proteins from the cell.
• Anti-Vimentin and anti-GAPDH antibodies are provided as markers for cytoskeleton and cytosol, respectively, in western blot analysis
• This kit will greatly increase the ability to detect and study the low abundance actin-associated proteins which are typically masked in conventional methods of Western blotting analysis of whole cell lysates

Specifications

• Content And Storage: The kit may be used for up to 4 months after receipt. Avoid repeated freeze/thaw cycles, and aliquot if necessary
• For Use With (Application): Immunofluorescence, Immunocytochemistry
• Quantity: 32 Assays/Kit