Invitrogen™ PSMD3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1974

Catalog No. PA1974

Description

PA1-974 detects proteasome 19S subunit S3 from human and mouse cells. PA1-974 has been successfully used in ICC/IF, FACS, and Western blot procedures. By Western blot, this antibody detects an ~60 kDa protein representing proteasome 19S subunit S3 from HeLa cell extract. For Western blot procedures it is necessary to transfer the protein to membrane at pH 11. PA1-974 immunizing peptide corresponds to amino acid residues 513-534 from human proteasome 19S subunit S3 protein.

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid.

Specifications

• Antigen: PSMD3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PSMD3
• Gene Alias: 26S proteasome non-ATPase regulatory subunit 3; 26S proteasome regulatory subunit RPN3; 26S proteasome regulatory subunit S3; AI255837; AntP91a; D11Bwg1349e; EGK_08646; P58; P91a; proteasome (prosome, macropain) 26S subunit, non-ATPase, 3; proteasome 26S non-ATPase subunit 3; proteasome 26S subunit, non-ATPase 3; proteasome subunit p58; Psd3; Psmd3; RPN3; S3; tissue specific transplantation antigen 2; tissue specific transplantation antigen P91A; transplantation antigen P91A; TSTA2; Tstap91a; Tum-P91A antigen
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22123, 5709
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: O43242, P14685
• Gene Symbols: PSMD3
• Immunogen: Synthetic Peptide: E(513) R E Q Q D L E F A K E M A E D D D D S F P(534).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG