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Molecular Probes™ Qdot™ 625 Streptavidin Conjugate Non-distribution product as customer accommodation.

Qdot™ 625 Streptavidin Conjugate

Manufacturer:  Molecular Probes™ A10196

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Catalog No. A10196


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Description

Description

Qdot™ 625 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot™ nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot™ nanocrystal conjugates are provided as 1µM solutions.

Important Features of Qdot™ Streptavidin Conjugates:
  • Streptavidin Qdot™ 625 conjugate has emission maxima of ~625 nm
  • Approximately 5 to 10 streptavidins per Qdot™ nanocrystal
  • Extremely photostable and bright fluorescence
  • Efficiently excited with single-line excitation sources
  • Narrow emission, large Stokes shift
  • Available in multiple colors
  • Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

    Properties of Qdot™ Nanocrystals
    The Qdot™ streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot™ streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot™ 705 and Qdot™ 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

    Other Fluorescent Conjugates of Streptavidin are Available
    We offer several other Qdot™ colors or try the Qdot™ Streptavidin Sampler Kit, containing Qdot™ streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor™ dyes, Oregon Green™ dye, enzyme conjugates, and traditional fluorophores like Texas Red™ dye, fluorescein (FITC) and more.

    Find Biotinylated Conjugates
    We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
  • Use the primary antibody search tool to find biotinylated primary antibodies
  • Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

    Blocking Endogenous Biotin
    Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

    Related Links:
    Learn more about Avidin-Biotin Detection
    Learn more about Qdot™ Nanocrystals
  • Specifications

    Specifications

    Very high binding affinity for biotin
    1μm
    Qdot™ 625 Streptavidin Conjugate, 200μL, Red, Liquid Form, 1μL Concentration, 76% Quantum Yield, Used in Western Blots, Flow Cytometry, Imaging and Microscopy, Microplate Assays, 2 to 8°C Storage Temperature
    Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
    Liquid
    Qdot™ 625 Streptavidin Conjugate
    200μL
    200μL
    2 to 8°C
    1 Each
    Red
    Qdot™ 625
    Fluorescent
    Liquid
    Nanometer-scale crystal
    Qdot™ 625 Streptavidin Conjugate
    For research use only
    200μL
    200μL
    1M betaine, 50mM borate, pH 8.3 with 0.05% Sodium azide
    Safety and Handling

    Safety and Handling

    ShelfLife : 6 Month(s)

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    For Research Use Only. Not for use in diagnostic procedures.