Molecular Probes Qdot 625 Streptavidin Conjugate
Qdot 625 Streptavidin Conjugate
Manufacturer: Molecular Probes A10196
DescriptionQdot 625 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot nanocrystal conjugates are provided as 1µM solutions.
Important Features of Qdot Streptavidin Conjugates:
Properties of Qdot Nanocrystals
The Qdot streptavidin conjugate is the size of a large macromolecule or protein (~1520 nm) and represents the brightest class of streptavidin detection reagents. Qdot streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot 705 and Qdot 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.
Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot colors or try the Qdot Streptavidin Sampler Kit, containing Qdot streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor dyes, Oregon Green dye, enzyme conjugates, and traditional fluorophores like Texas Red dye, fluorescein (FITC) and more.
Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
Learn more about Avidin-Biotin Detection
Learn more about Qdot Nanocrystals
|Very high binding affinity for biotin|
|Qdot™ 625 Streptavidin Conjugate, 200μL, Red, Liquid Form, 1μL Concentration, 76% Quantum Yield, Used in Western Blots, Flow Cytometry, Imaging and Microscopy, Microplate Assays, 2 to 8°C Storage Temperature|
|Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more|
|Qdot™ 625 Streptavidin Conjugate|
|2 to 8°C|
|Qdot™ 625 Streptavidin Conjugate|
|For research use only|
|1M betaine, 50mM borate, pH 8.3 with 0.05% Sodium azide|
Safety and Handling
ShelfLife : 6 Month(s)
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For Research Use Only. Not for use in diagnostic procedures.