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  5. Invitrogen™ Quant-iT™ 1X dsDNA Assay Kits, high sensitivity (HS) and broad range (BR) 

Invitrogen™ Quant-iT™ 1X dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
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Catalog No. Q33267
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Quantity:
1 kit
1000 assays
Assay:
1 x dsDNA, broad range
1 x dsDNA, high sensitivity
Quantitation Range:
0.2 to 100 ng
4 to 4000 ng
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Catalog No. Quantity Assay Quantitation Range
Q33267 1 kit 1 x dsDNA, broad range 4 to 4000 ng
Q33232 1000 assays 1 x dsDNA, high sensitivity 0.2 to 100 ng
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Catalog No. Q33267 Supplier Invitrogen™ Supplier No. Q33267
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Description

For easy and accurate dsDNA quantitation, the Quant-iT 1X dsDNA HS and BR Assays enable up to 1,000 fluorescence-based assays in a 96-well microplate format.

Make DNA quantitation easy and accurate in a ready-to-go 1X formulation with the Quant-iT 1X dsDNA HS (High-Sensitivity) and Quant-iT 1X dsDNA BR (Broad Range) Assay kits. Both kits provide a ready-to-use working solution. Simply load your sample, add the Qubit 1X dsDNA HS or BR working solution, and read the concentration using a fluorescence-based microplate reader.

The Quant-iT 1X dsDNA HS and BR Assay kits are highly selective for double-stranded DNA (dsDNA) over RNA, providing an accurate and precise method for the quantification of dsDNA samples. To perform the assay, dilute your sample (any volume from 1–20 μL is acceptable) into the 1X working solution provided, then read the concentration using a fluorescence plate reader.

The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/μL to 100 ng/μL, providing a core detection range of 0.2 ng to 100 ng of DNA in the assay tube. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/μL to 4000 ng/μL (depending on sample volume), providing a detection range of 4–4000 ng of DNA.

Common contaminants such as salts, free nucleotides, solvents, detergents, and protein are well tolerated in the dsDNA assays. The '1X' dsDNA assay kits provide reagent and buffer in a ready-to-use formulation and are highly selective for dsDNA over ssDNA, RNA, and protein.

For Research Use Only. Not for use in diagnostic procedures.

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Specifications

Assay 1 x dsDNA, broad range
Detection Method Fluorescence
Excitation/Emission 506/523
For Use With (Equipment) Microplate Reader
No. of Reactions 1000 (200 μL Assay Volume)
Quantitation Range 4 to 4000 ng
Product Line Quant-iT
Quantity 1 kit
Shipping Condition This assay kit will ship as two components.
• Quant-iT 1 x dsDNA BR Working Solution will be shipped (at ambient conditions.)
• Quant-iT 1 x dsDNA BR lambda DNA standards will be shipped on wet ice.
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