Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Description

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

Thermo Scientific™ QuantaBlu Fluorogenic Peroxidase Substrate is a soluble fluorogenic (chemifluorescent) substrate for detection of peroxidase activity in ELISA methods.

Additional Information and Online Ordering

In the presence of active peroxidase the working solution of QuantaBlu Substrate produces a blue fluorescent product that can be quantitated by fluorometry in microplates or cuvettes. Fluorometric-based detection has a large dynamic range, which overcomes the limitations of using spectrophotometry to measure colorimetric substrates. The blue fluorescent product is not sensitive to light and does not photobleach. QuantaBlu Fluorogenic Peroxidase Substrate is stable, highly sensitive, produces high signal-to-noise ratios and allows for a broad dynamic detection range of peroxidase activity. This substrate is also flexible and can be used for stopped, nonstopped and kinetic assays. Incubation for stopped and nonstopped assays can be performed between 5 and 90 minutes at either room temperature (RT) or 37°C. The QuantaBlu NS/K Substrate formulation can be integrated into robotic-based assays and is designed for assays that do not need to be stopped.

Highlights:

• More sensitive than TMB, OPD or ABTS substrates
• Flexible stopped, nonstopped or kinetic assays possible
• Large dynamic range (4 log peroxidase concentration range)
• Excellent stability; working solution is stable for 24 hours
• Large stokes shift; excitation/emission maxima of 325nm/420nm (range of 315-345/370-460)
• Does not photobleach

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

• Description: QuantaBlu Fluorogenic Peroxidase Substrate
• Product Type: ELISA Substrate
• Quantity: 530 mL
• Detection Method: Fluorescent
• Product Line: QuantaBlu™
• Substrate Properties: Chemical Substrate
• Substrate Type: HRP (Horseradish Peroxidase)
• Content And Storage: Sufficient For: 275 mL of 1X working solution for non-stopped assay (2750 wells at 100 μL per well) or 250 mL of 1X working solution when used 1:1 with stop solution (2500 wells at 100 μL per well)
  • QuantaBlu Substrate Solution, 250 mL
  • QuantaBlu Peroxide Solution, 30 mL
  • QuantaBlu Stop Solution, 250 mL
  Store at 4°C.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.