Applied Biosystems™ QuantStudio™ 3D Digital PCR 20K Chip Kit v2 and Master Mix

Catalog No.
A26317	QuantStudio™ 3D Digital PCR 20K Chip Kit v2 and Master Mix

Catalog No. A26317 Supplier Applied Biosystems™ Supplier No. A26317

Description

The Applied Biosystems™ QuantStudio™ 3D Digital PCR 20K Chip Kit v2 and QuantStudio™ 3D Digital PCR Master Mix v2 deliver improved and UV adhesive-free sealing.

The Applied Biosystems™ QuantStudio™ 3D Digital PCR 20K Chip Kit v2 and QuantStudio™ 3D Digital PCR Master Mix v2 are fully-compatible with all QuantStudio 3D™ Digital PCR Instrument hardware and software.

The QuantStudio™ 3D Digital PCR 20K Chip Kit v2 is a key component of the QuantStudio™ 3D Digital PCR System. Manufactured from a silicon substrate and etched with 20,000 consistently-sized nanoscale reaction wells, the chip enables precise sample loading with an uncomplicated workflow. It requires use of the QuantStudio 3D Digital PCR Master Mix v2.

QuantStudio 3D Digital PCR Master Mix v2 is specifically formulated with all reagents required to help ensure robust PCR amplification of single copy templates. It is optimized for use with isolated DNA or cDNA, TaqMan™ assays, and the QuantStudio 3D Digital PCR 20K Chip v2.

The QuantStudio 3D Digital PCR 20K Chip Kit v2 and QuantStudio 3D Digital PCR Master Mix v2 are designed to deliver accurate, sensitive, and precise digital PCR data on QuantStudio 3D Digital PCR instruments when combined with associated reagents and QuantStudio™ 3D Analysis Suite™ software.

Specifications

• Content And Storage: 8 x 12 QuantStudio 3D Digital PCR 20K Chips V2, 8 x 12 QuantStudio 3D Digital PCR Chip Lids V2, 8 x12 QuantStudio 3D Digital PCR Sample Loading Blades, 8 x 3 Syringes Immersion Fluid, 8 x 3 Immersion Fluid Tips, 1.5 mL QuantStudio 3D Digital PCR Master Mix V2
• For Use With (Equipment): QuantStudio™ 3D
• Product Line: QuantStudio
• Shipping Condition: Dry Ice
• Quantity: 8 x 12 chips

Frequently Asked Questions (FAQs)

How do I replace the fuses in the QuantStudio 3D Digital PCR System?

Please follow the procedures described in "Chapter A: Maintenance" of the QuantStudio 3D Digital PCR System User Guide to replace the fuses. Make sure to only replace with those listed and certified fuses of the same type and rating as those currently in the QuantStudio 3D instrument.

How does sample preparation for digital PCR differ from real-time PCR?

The answer is that sample prep for digital PCR is no different from real-time PCR when using the QuantStudio 3D Digital PCR System. If you have samples in your freezer that you've run on real-time PCR, then you're ready to run them with digital PCR.

How do I determine how much sample to use when setting up the digital PCR reaction for the QuantStudio 3D Digital PCR System?

Please see the section on Sample Dilution in Chapter 3 of the QuantStudio 3D Digital PCR System User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf) for more details on how to determine the target copy number per genome.

Where are my chip image data saved on the QuantStudio 3D instrument?

You can configure the QuantStudio 3D instrument to upload the imaging data to a USB drive, a network file server, or the cloud data storage service. See Appendix B, “Networking”, in the QuantStudio 3D Digital PCR System User Guide for more information on setting up network destinations. Also, the QuantStudio 3D instrument retains a copy of the analyzed data from the imaged QuantStudio 3D Digital PCR 20K Chip that you can access from the Run History screen. Note: The results will remain in the QuantStudio 3D instrument cache for approximately 300 readings. After 300 chips, the instrument removes the oldest data file in the cache to store each new reading.

Is there any way to access the raw fluorescence data from the QuantStudio 3D Chips in AnalysisSuite software v3.0?

Yes, there is an option to export the Chip data as .xml on the Export tab in AnalysisSuite software v3.0.

What is the advantage of using the new Poisson Plus algorithm in AnalysisSuite software v3.0?

The Poisson Plus algorithm corrects for well-to-well load volume variation on a per Chip basis. This becomes important at higher target concentrations.

When should I use the new Poisson Plus algorithm in AnalysisSuite software v3.0?

You should use the Poisson Plus algorithm for projects that contain QuantStudio 3D Chips with target quantities >2,000 copies/µL.

Can I purchase Immersion Fluid separately for the QuantStudio 3D Digital PCR System?

Yes, please use Cat. No. A27322.

In the QuantStudio 3D Digital PCR System, how does well-to-well volume variation affect data?

At low concentrations, there is no impact on the data. At higher concentrations (>2,000 copies/µL), there is a slight impact. Using the Poisson Plus algorithm will correct for well-to-well volume variation.

Are there any differences in well-to-well volume variation between the QuantStudio 3D Digital PCR v1 and v2 Chips?

No significant difference has been measured.

Can current QuantStudio 3D Digital PCR v1 Chips be mixed in a project with the new v2 Chips?

Yes, internal studies have shown comparable performance between v1 and v2 Chips.

Can I use QuantStudio 3D Firmware v3.0 to read older Chips/Lids?

Yes, Firmware v3.0 will work with all versions of Chips and Lids.

Can I use QuantStudio 3D Firmware v2.0 or v1.1 to analyze the new v2 Chip?

No. No matter which version of Chip Lid is being used, v2 Chips require Firmware v3.0 for proper data analysis.

Can I use QuantStudio 3D Firmware v2.0 or v1.1 to read the new v2 Chip Lids with 2D barcodes?

We recommend that you upgrade the firmware to v3.0 in order to read the 2D barcode; otherwise the .eds files will not have a Chip ID.

Can I use the QuantStudio 3D Digital PCR Master Mix v2 with the current v1 Chips?

No, the v2 Master Mix can only be used with the v2 Chips.

Can I use the current v1 QuantStudio 3D Digital PCR Master Mix with the new v2 Chips?

No, the current v1 Master Mix can only be used with the older Chips.

I have a lot of the current v1 Applied Biosystems QuantStudio 3D Digital PCR Chips; can they be used with the new v2 Chip Lids?

Yes, the current Chips will work with the new v2 Chip Lids.

I have a lot of the current v1 Applied Biosystems QuantStudio 3D Digital PCR Chip Lids, can they be used with the new v2 Chips?

Yes, the current Lids will work on the new v2 Chips.

For Research Use Only. Not for use in diagnostic procedures.