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Invitrogen™ Qubit™ RNA IQ Assay Kits

Obtain ready-to-use detection of RNA degradation information with the Qubit RNA integrity and quality (IQ) Assay Kit and Standards, which feature two unique dyes for binding large and/or highly structured RNA and small, degraded RNA. Together, these dyes enable detection of RNA quality and integrity.

Manufacturer:  Invitrogen™ Q33221

Encompass

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Catalog No. Q33221


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Description

Description

Quickly and easily determine RNA degradation with the Qubit RNA IQ Assay Kit and Qubit RNA IQ Standards.  RNA integrity and quality (IQ) information is assessed using the assay kit’s two unique dyes: one dye that binds to large and/or highly structured RNA and a second dye that selectively binds to small, degraded RNA.  Together, the dyes enable you to quickly assess the quality and integrity of your RNA samples, with results presented as an RNA IQ number and as a calculated percent of large and small RNA in the sample.

Qubit RNA IQ Assay Kit
The Qubit RNA IQ Assay Kit provides a fast, simple method to check whether an RNA sample has degraded using a Qubit 4 or Qubit Flex fluorometer. The RNA IQ assay utilizes two unique dyes: one that binds to large, intact, and/or highly structured RNA (mRNA, tRNA, rRNA), and another that selectively binds to small and/or degraded RNA. Together they enable you to quickly assess the quality and integrity of the RNA sample.

To use, simply add your samples to the RNA IQ working solution, then measure the signal on a Qubit 4 or Qubit Flex fluorometer. Results are presented as an RNA IQ number (RNA IQ#) that indicates the RNA sample integrity and quality. Results are also posted as a calculated percent of large and small RNA in the sample. The RNA IQ# is a value from 1 to 10, similar to other RNA quality scores, where a small number indicates that the sample is comprised of mainly small RNA and a larger number indicates that the sample consists of mainly large RNA or RNA with tertiary structure.

Other features of the Qubit RNA IQ Assay Kit
• 5S and tRNA tertiary structure binds large RNA dye
• Accuracy even in the presence of salts, protein, solvents, and RNA stabilization reagents
• Signal stable for 1 hour
• For use with the Qubit 4 Fluorometer only; does not work on original Qubit, Qubit 2.0, or Qubit 3 fluorometers

Solution-based chemistry in line with electrophoresis
The RNA IQ assay results correlate well with electrophoretic methods. The primary difference between the solution-based RNA IQ assay and traditional electrophoresis-based techniques is the amount of time it takes to ascertain whether an RNA sample has degraded. Gels are considered easy to perform, but still take time. Microfluidic-based methods not only take time to obtain results, but often have lengthy procedures to prepare the samples. Gel or microfluidic-based electrophoresis is still recommended for obtaining fragment size information or size distribution of the RNA sample.

Qubit RNA IQ Assay Standards
The Qubit RNA IQ Assay RNA standards are designed for use with the Qubit RNA IQ Assay Kit. Sufficient amounts of the small and large and/or structured RNA standard are supplied for generating an IQ standard curve for up to 500 samples. The RNA IQ standards are designed to measure RNA degradation and are not interchangeable with the standards from other RNA quantitation assays, including the Qubit RNA HS, BR, or XR assay kits.

Notes:
1. 500 µL thin-walled PCR tubes (Cat. No. Q32856) are required for all Qubit assays and are not included with these Qubit assay standards.
2. The Qubit RNA IQ Assay will only work on a Qubit 4 or Qubit Flex fluorometer.
3. The assay is not designed for use with Qubit, Qubit 2.0, or Qubit 3 fluorometers.
4. Use thin-wall, clear, 0.5 mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200 µL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer.
Specifications

Specifications

Qubit RNA Integrity and Quality (IQ) Assay Kit
Qubit
Qubit 4 and Qubit Flex Fluorometer
75 Assays
Dry Ice
SDS
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