R&D Systems™ 3'-Phosphoadenosine-5'-phosphosulfate

Substrate for sulfotransferase assay

Supplier:  R&D Systems™ ES019

Catalog No. ES019

Description

3'-Phosphoadenosine-5'-phosphosulfate (PAPS) is the universal sulfur donor for sulfotransferases. It is synthesized from ATP through two steps. In the first step, one ATP molecule is converted to adenosine-5'-phosphosulfate (APS) by ATP sulfurylase and pyrophosphate (PPi) is generated as a byproduct. In the second step, the APS is converted to PAPS by APS kinase and ADP is generated as a byproduct. To drive the reaction forward, the pyrophosphate can be further degraded to phosphate (Pi) by inorganic pyrophosphatase to eliminate feedback inhibition, and the ADP can be converted back to ATP using pyruvate kinase.

• Suitable for kinetic assay of sulfotransferases using the Universal Sulfotransferase Activity Kit (Mfr. No. EA003) or for enzymatic synthesis of sulfonated molecules
• Purity >90% of the nucleotide based on HPLC: No Li+ or Na+ ions, and free inorganic phosphate <0.5%

Specifications

• Quantity: 500 μg
• Product Type: PAPS, C10H15N5O13P2S
• Storage Requirements: Store the unopened product at -70° C for up to six months. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.