R&D Systems™ Bacterial Thermolysin Protein, CF

Catalog No.	Quantity
3097ZN020	20 μg

Catalog No. 3097ZN020 Supplier R&D Systems™ Supplier No. 3097ZN020

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005 ). The specific activity is >12,000 pmol/min/μg, as measured with 10 μM ES005 and 2 ng enzyme in 100 μL of 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij 35, pH 7.5.

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Specifications

• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
• Molecular Weight (g/mol): M.W. (Observed): 36 kDa, reducing conditions
• Name: Thermolysin
• Quantity: 20 μg
• Source: Geobacillus stearothermophilus -derived Thermolysin protein
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Purity or Quality Grade: >70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Thermolysin