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  6. R&D Systems™ Cultrex 3-D Culture Matrix RGF Basement Membrane Extract 

R&D Systems™ Cultrex 3-D Culture Matrix RGF Basement Membrane Extract
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Catalog No. 344500501
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5 mL
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344500501 5 mL
344500101 1 mL
344501001 2 x 5 mL
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Description

Applications: Bioactivity

Functional assays: Tube formation assay - BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells. 3D Culture - Basement Membrane Extract promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) or human prostate (PC-3) into acinar structures. Gelling assay: BME gels in less than 30 minutes at 37degreesC, and maintains the gelled form in culture medium for a minimum of 14 days at 37degreesC.

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Specifications

Concentration 8-12 mg/mL (by Lowry assay)
For Use With (Application) Bioactivity
Quantity 5 mL
Endotoxin Level ≤8EU/mL
Source Murine Engelbreth-Holm-Swarm (EHS) tumor.
Content And Storage Product is stable for a minimum of 3 months from date of shipment when stored at -20° C in a manual defrost freezer. For optimal stability, store at -80° C. Avoid freeze-thaw cycles.
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