R&D Systems™ Cultrex RGF Basement Membrane Extract, Type 2

Catalog No.	Quantity
35-330-0502	5 mL
35-330-0102	1 mL
35-330-1002	2 x 5 mL

Catalog No. 35-330-0502 Supplier R&D Systems™ Supplier No. 353300502

Description

For Robust Organoid Cultures - Reduced Growth Factor Basement Membrane Extract

Functional Assay - Tube formation assay: BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells. Gelling Assay - BME gels in less than 30 minutes at 37°C, and maintains the gelled form in culture medium for a minimum of 14 days at 37°C.

Specifications

• Concentration: 8-12 mg/mL (by Lowry assay)
• For Use With (Application): Bioactivity
• Quantity: 5 mL
• Source: Murine Engelbreth-Holm-Swarm (EHS) tumor.
• Content And Storage: Product is stable for a minimum of 3 months from date of shipment when stored at -20°C in a manual defrost freezer. For optimal stability, store at -80°C. Avoid freeze-thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 μg/mL gentamicin sulfate. Sterility Testing: PathClear™ - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation (at 37°C for 14 days following USP sterility testing guidelines.)