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R&D Systems™ Cultrex RGF Basement Membrane Extract, Type 2 Available on GSA/VA Contract for Federal Government customers only.

For Robust Organoid Cultures - Reduced Growth Factor Basement Membrane Extract

Manufacturer:  R&D Systems™ 353300502

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Catalog No. 35-330-0502

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Functional Assay - Tube formation assay: BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells. Gelling Assay - BME gels in less than 30 minutes at 37°C, and maintains the gelled form in culture medium for a minimum of 14 days at 37°C.

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5 mL
Product is stable for a minimum of 3 months from date of shipment when stored at -20°C in a manual defrost freezer. For optimal stability, store at -80°C. Avoid freeze-thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 µg/mL gentamicin sulfate. Sterility Testing: PathClear™ - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation (at 37°C for 14 days following USP sterility testing guidelines.)
12 - 18 mg/mL
Murine Engelbreth-Holm-Swarm (EHS) tumor.
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